{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:18:45Z","timestamp":1770329925483,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["P30 NS061800"],"award-info":[{"award-number":["P30 NS061800"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sympathetic neurons that innervate the heart are located primarily in the stellate ganglia (SG), which also contains neurons that project to brown adipose tissue (BAT). These studies were designed to examine the morphology of these two populations (cardiac\u2010 and BAT\u2010projecting) and their target connectivity. We examined SG neurons in C57BL\/6J mice following injections of the retrograde tracer cholera toxin B (CTb) conjugated to Alexa Fluor 488 and Alexa Fluor 555, into cardiac tissue and intrascapular BAT. BAT\u2010projecting SG neurons were widely dispersed in SG, while cardiac\u2010projecting SG neurons were localized primarily near the inferior cardiac nerve base. SG neurons were not dual\u2010labeled, suggesting that sympathetic innervation is specific to the heart and BAT, supporting the idea of \u201clabeled lines\u201d of efferents. Morphologically, cardiac\u2010projecting SG somata had more volume and were less abundant than BAT\u2010projecting neurons using our tracer\u2010labeling paradigm. We found a positive correlation between the number of primary dendrites per neuron and soma volume in cardiac\u2010projecting SG neurons, though not in BAT\u2010projecting neurons. In both SG subpopulations, the number of cholinergic inputs marked with vesicular acetylcholine transporter (VAChT) puncta contacting the soma was positively correlated to soma volume, suggesting scaling of inputs across a range of neuronal sizes. In separate studies using dual tracing from left and right BAT, we found that BAT\u2010projecting SG neurons were located predominately ipsilateral to the injection, but a small subset of SG neurons project bilaterally to BAT. This tracing approach will allow the assessment of cell\u2010specific mechanisms of plasticity within subpopulations of SG neurons.<\/jats:p>","DOI":"10.14814\/phy2.15334","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T09:25:11Z","timestamp":1653643511000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Distinct morphology of cardiac\u2010 and brown adipose tissue\u2010projecting neurons in the stellate ganglia of mice"],"prefix":"10.14814","volume":"10","author":[{"given":"Madeleine S.","family":"Barrett","sequence":"first","affiliation":[{"name":"Department of Chemical Physiology &amp; Biochemistry Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah M.","family":"Hegarty","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology &amp; Biochemistry Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4658-8730","authenticated-orcid":false,"given":"Beth A.","family":"Habecker","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology &amp; Biochemistry Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5310-4055","authenticated-orcid":false,"given":"Sue A.","family":"Aicher","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology &amp; Biochemistry Oregon Health &amp; Science University  Portland Oregon 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