{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T06:43:34Z","timestamp":1756190614777,"version":"3.41.2"},"reference-count":64,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T00:00:00Z","timestamp":1662422400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic kidney disease (CKD) is a priority health problem affecting 36% of Egyptians. Adipose\u2010derived mesenchymal stem cells (ADMSCs) have multidifferentiation capacity and the ability to restore several types of cells including damaged renal cells. Granulocyte colony\u2010stimulating factor (G\u2010CSF) is known to mobilize hematopoietic stem cells from bone marrow to the peripheral circulation. The aim of this study was to compare the effect of endogenous CD34<jats:sup>+<\/jats:sup> cells mobilization and exogenous ADMSCs administration in the treatment of a rat model of adriamycin (ADR)\u2010induced CKD. A total of 48 male albino rats of the local strain (200\u2009\u00b1\u200950\u2009g) were equally divided into four groups: control negative, ADR (control positive), ADMSCs group, and G\u2010CSF group. Six rats from each group were sacrificed after 4\u2009weeks and the other 6 after 12\u2009weeks. Renal function was assessed frequently by measuring serum creatinine, albumin, urea, 24\u2010h urinary protein level, and hemoglobin level throughout the study. Oxidative stress markers malondialdehyde (MDA) and total antioxidant (TAO) were measured on day 28. CD\u201034<jats:sup>+<\/jats:sup> cell percentage was measured on day 9. After the sacrification of the rats, kidneys were removed for histopathological assessment. Results revealed that both ADMSCs and G\u2010CSF significantly improved serum creatinine, albumin, urea, 24\u2010h urinary protein level, and histopathological damage score, with the G\u2010CSF\u2010treated group showing better improvement in 24\u2010h urinary protein level, serum albumin, and histopathological damage score compared with ADMSCs\u2010treated group. The G\u2010CSF group also had significantly higher levels of CD34<jats:sup>+<\/jats:sup> cells. Oxidative stress markers (MDA and TAO) levels were significantly improved with both therapies. We conclude that mobilization of endogenous hematopoietic stem cells by G\u2010CSF is more effective than exogenously injected ADMSCs in protecting the kidneys against AD\u2010induced toxicity.<\/jats:p>","DOI":"10.14814\/phy2.15448","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T09:25:24Z","timestamp":1662456324000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease?"],"prefix":"10.14814","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1066-4949","authenticated-orcid":false,"given":"Shereen","family":"Morsy","sequence":"first","affiliation":[{"name":"Physiology Department, Faculty of Medicine Suez Canal University  Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8021-5348","authenticated-orcid":false,"given":"Mona F.","family":"Mansour","sequence":"additional","affiliation":[{"name":"Physiology Department, Faculty of Medicine Suez Canal University  Ismailia Egypt"},{"name":"Centre of Excellence in Molecular and Cellular Medicine, Faculty of Medicine Suez Canal University  Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9969-7789","authenticated-orcid":false,"given":"Mohamed","family":"Abdo","sequence":"additional","affiliation":[{"name":"Physiology Department, Faculty of Medicine Suez Canal University  Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4034-0213","authenticated-orcid":false,"given":"Yasser","family":"El\u2010Wazir","sequence":"additional","affiliation":[{"name":"Physiology Department, Faculty of Medicine Suez Canal University  Ismailia Egypt"},{"name":"Centre of Excellence in Molecular and Cellular Medicine, Faculty of Medicine Suez Canal University  Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,6]]},"reference":[{"issue":"4","key":"e_1_2_9_2_1","first-page":"197","article-title":"Histological study on the protective effect of endogenous stem cell mobilization in busulfan\u2010induced testicular injury in albino rats","volume":"6","author":"Aboul Fotouh G. 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