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SN stimulation is a novel approach to electrically modulating bladder function. In previous animal studies, bladder\u2010inhibitory responses were evoked by low\u2010amplitude (25\u2009\u03bcA) stimulus pulses applied in short\u2010duration (10\u00a0min) trials and at frequencies between 10 and 20\u2009Hz. Experiments were performed in urethane\u2010anesthetized rats that were separated into three groups: intravesical saline infusion\u2009+\u2009SN stimulation (group A), intravesical 0.1% acetic acid infusion\u2009+\u2009SN stimulation (group B), and intravesical saline infusion\u2009+\u2009no SN stimulation (group C). Changes in bladder function\u2014 basal bladder pressure (<jats:italic>P<\/jats:italic>\u2009<jats:sub>base<\/jats:sub>), contraction amplitude (\u0394<jats:italic>P<\/jats:italic>), and inter\u2010contraction interval (<jats:italic>T<\/jats:italic>\u2009<jats:sub>ICI<\/jats:sub>)\u2014were measured in response to stimulation trials applied for different durations (10, 20, and 40\u2009min). Trials were also repeated at frequencies of 10 and 20\u2009Hz. In group A, longer\u2010duration (40\u2009min) stimulation trials applied at 10\u00a0Hz evoked overflow incontinence (OI) episodes that were characterized by significant changes in <jats:italic>P<\/jats:italic>\u2009<jats:sub>base<\/jats:sub> (122.7\u2009\u00b1\u20099.1%, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.026), \u0394<jats:italic>P<\/jats:italic> (\u221260.8\u2009\u00b1\u200912.8%, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.044), and <jats:italic>T<\/jats:italic>\u2009<jats:sub>ICI<\/jats:sub> (\u221243.2\u2009\u00b1\u200913.0%, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.031). Stimulation\u2010evoked OI was observed in 5 of 8 animals and lasted for 56.5\u2009\u00b1\u200910.7\u00a0min. In contrast, no significant changes in bladder function were observed in either group B or group C. Our findings show that longer\u2010duration trials consisting of electrical pulses applied at 10\u00a0Hz are important stimulation parameters that elicit inhibitory bladder responses in anesthetized rodents.<\/jats:p>","DOI":"10.14814\/phy2.15517","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T07:31:08Z","timestamp":1669102268000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prolonged inhibition of bladder function is evoked by low\u2010amplitude electrical stimulation of the saphenous nerve in urethane\u2010anesthetized rats"],"prefix":"10.14814","volume":"10","author":[{"given":"Zainab","family":"Moazzam","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering (BME) University of Toronto  Ontario 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