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This study aimed to utilize urinary biomarkers to characterize AKI in an established neonatal rat model of HIE. Day 7 Sprague\u2013Dawley rat pups underwent HIE using the Rice\u2013Vannucci model (unilateral carotid ligation followed by 120 mins of 8% oxygen). Controls included no surgery and sham surgery. Weights and urine for biomarkers (NGAL, osteopontin, KIM\u20101, albumin) were collected the day prior, daily for 3\u2009days post\u2010intervention, and at sacrifice day 14. Kidneys and brains were processed for histology. HIE pups displayed histological evidence of kidney injury including damage to the proximal tubules, consistent with resolving acute tubular necrosis, and had significantly elevated urinary levels of NGAL and albumin compared to sham or controls 1\u2010day post\u2010insult that elevated for 3\u2009days. KIM\u20101 significantly increased for 2\u2009days post\u2010HIE. HIE did not significantly alter osteopontin levels. Seven days post\u2010start of experiment, controls were 81.2% above starting weight compared to 52.1% in HIE pups. NGAL and albumin levels inversely correlated with body weight following HIE injury. The AKI produced by the Rice\u2013Vannucci HIE model is detectable by urinary biomarkers, which can be used for future studies of treatments to reduce kidney injury.<\/jats:p>","DOI":"10.14814\/phy2.15533","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T10:10:21Z","timestamp":1671617421000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Neonatal hypoxic ischemic encephalopathy increases acute kidney injury urinary biomarkers in a rat model"],"prefix":"10.14814","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1810-5532","authenticated-orcid":false,"given":"Angela M.","family":"Groves","sequence":"first","affiliation":[{"name":"Department of Pediatrics University of Rochester School of Medicine and Dentistry New York Rochester USA"},{"name":"Department of Radiation Oncology University of Rochester School of Medicine and Dentistry New York Rochester 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