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The aim of this study was to investigate the effects of sympathetic nerve stimulation on three different neurogenic motor behaviors of the distal colon: transient neural events (TNEs), colonic motor complexes (CMCs), and pellet propulsion. To do this, segments of guinea pig distal colon with intact connections to the inferior mesenteric ganglion (IMG) were set up in organ baths allowing for simultaneous extracellular suction electrode recordings from smooth muscle, video recordings for diameter mapping, and intraluminal manometry. Electrical stimulation (1\u201320\u2009Hz) of colonic nerves surrounding the inferior mesenteric artery caused a statistically significant, frequency\u2010dependent inhibition of TNEs, as well as single pellet propulsion, from frequencies of 5\u00a0Hz and greater. Significant inhibition of CMCs required stimulation frequencies of 10\u00a0Hz and greater. Phentolamine (3.6\u00a0\u03bcM) abolished effects of colonic nerve stimulation, consistent with a sympathetic noradrenergic mechanism. Sympathetic inhibition was constrained to regions with intact extrinsic nerve pathways, allowing normal motor behaviors to continue without modulation in adjacent extrinsically denervated regions of the same colonic segments. The results demonstrate differential sensitivities to sympathetic input among distinct neurogenic motor behaviors of the colon. Together with findings indicating CMCs activate colo\u2010colonic sympathetic reflexes through the IMG, these results raise the possibility that CMCs may paradoxically facilitate suppression of pellet movement in vivo, through peripheral sympathetic reflex circuits.<\/jats:p>","DOI":"10.14814\/phy2.15567","type":"journal-article","created":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T07:32:12Z","timestamp":1673595132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Stimulation of extrinsic sympathetic nerves differentially affects neurogenic motor activity in guinea pig distal colon"],"prefix":"10.14814","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1817-0158","authenticated-orcid":false,"given":"David J.","family":"Smolilo","sequence":"first","affiliation":[{"name":"College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3747-140X","authenticated-orcid":false,"given":"Timothy J.","family":"Hibberd","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1082-7349","authenticated-orcid":false,"given":"Marcello","family":"Costa","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8910-2801","authenticated-orcid":false,"given":"Phil G.","family":"Dinning","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"},{"name":"Department of Surgery Flinders Medical Centre  Bedford Park South Australia Australia"},{"name":"Department of Gastroenterology Flinders Medical Centre  Bedford Park South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren J.","family":"Keightley","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dayan","family":"De\u00a0Fontgalland","sequence":"additional","affiliation":[{"name":"Department of Surgery Flinders Medical Centre  Bedford Park South Australia Australia"},{"name":"Department of Gastroenterology Flinders Medical Centre  Bedford Park South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David\u00a0A.","family":"Wattchow","sequence":"additional","affiliation":[{"name":"Department of Surgery Flinders Medical Centre  Bedford Park South Australia Australia"},{"name":"Department of Gastroenterology Flinders Medical Centre  Bedford Park South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2190-5303","authenticated-orcid":false,"given":"Nick J.","family":"Spencer","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1969.tb08298.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23704"},{"key":"e_1_2_8_4_1","first-page":"G749","article-title":"Neurogenic and myogenic motor activity in the colon of the guinea pig, mouse, rabbit, and rat. 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