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We and others previously reported sex differences in BP in normotensive and hypertensive rat models where females have lower BP than age\u2010matched males. As females are known to have greater NO bioavailability than age\u2010matched males, the current study was designed to test the hypothesis that anesthetized female normotensive Wistar Kyoto rats (WKY) are more responsive to acute NOS inhibition\u2010induced increases in BP compared to male WKY. Twelve\u2010week\u2010old male and female WKY were randomized to infusion of the nonspecific NOS inhibitor N<jats:sup>G<\/jats:sup>\u2010nitro\u2010L\u2010arginine methyl ester (L\u2010NAME, 1\u2009mg\/kg\/min) or selective NOS1 inhibition with vinyl\u2010L\u2010NIO (VNIO, 0.5\u2009mg\/kg\/min) for 60\u2009min. Mean arterial BP, glomerular filtration rate (GFR), urine volume, and electrolyte excretion were assessed before, and during L\u2010NAME or VNIO infusion. L\u2010NAME and VNIO significantly increased BP in both sexes; however, the increase in BP with L\u2010NAME infusion was greater in females versus males compared to baseline BP values. Acute infusion of neither L\u2010NAME nor VNIO for 60\u2009min altered GFR in either sex. However, urine volume, sodium, chloride and potassium excretion levels increased comparably in male and female WKY with L\u2010NAME and VNIO infusion. Our findings suggest sex differences in BP responses to acute non\u2010isoform\u2010specific NOS inhibition in WKY, with females being more responsive to L\u2010NAME\u2010induced elevations in BP relative to male WKY. However, sex differences in the BP response did not coincide with sex differences in renal hemodynamic responses to acute NOS inhibition.<\/jats:p>","DOI":"10.14814\/phy2.15771","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:07:36Z","timestamp":1691453256000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Acute nitric oxide synthase inhibition induces greater increases in blood pressure in female versus male Wistar Kyoto rats"],"prefix":"10.14814","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3065-4457","authenticated-orcid":false,"given":"Ahmed A.","family":"Elmarakby","sequence":"first","affiliation":[{"name":"Departments of Oral Biology &amp; Diagnostic Sciences Augusta University  Augusta Georgia USA"},{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2649-9850","authenticated-orcid":false,"given":"Karim M.","family":"Saad","sequence":"additional","affiliation":[{"name":"Departments of Oral Biology &amp; Diagnostic Sciences Augusta University  Augusta Georgia USA"},{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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