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The aim of this report was to examine heart rate variability threshold (HRVT) behavior across different ramp incremental (RI) slopes. Seventeen participants completed a series of three RI (15, 30, and 45\u2009W\u2009\u00b7\u2009min<jats:sup>\u22121<\/jats:sup> slopes) with monitoring of gas exchange parameters, heart rate (HR) and HRV. HRVT1 was defined as the V\u0307O<jats:sub>2<\/jats:sub> or HR at which DFA a1 reached 0.75 and the HRVT2 at which these values reached 0.5. HRVTs were compared by Pearson's <jats:italic>r<\/jats:italic>, Bland\u2013Altman analysis, ICC<jats:sub>3,1<\/jats:sub>, ANOVA, and paired <jats:italic>t<\/jats:italic>\u2010testing. An excellent degree of reliability was seen across all three ramps, with an ICC<jats:sub>3,1<\/jats:sub> of 0.93 and 0.88 for the HRVT1 V\u0307O<jats:sub>2<\/jats:sub> and HR, respectively, and 0.90 and 0.92 for the HRVT2 V\u0307O<jats:sub>2<\/jats:sub> and HR, respectively. Correlations between HRVT1\/2 of the individual ramps were high with <jats:italic>r<\/jats:italic> values 0.84\u20130.95 for both HR and V\u0307O<jats:sub>2<\/jats:sub>. Bland\u2013Altman differences ranged between \u22121.4 and 1.2\u2009mL\u2009\u00b7\u2009kg<jats:sup>\u22121<\/jats:sup>\u2009\u00b7\u2009min<jats:sup>\u22121<\/jats:sup> and \u22122 and +2\u2009bpm. Paired <jats:italic>t<\/jats:italic>\u2010testing showed no mean differences between any HRVT1\/2 ramp comparisons. Cycling ramp slope does not appear to affect either HRVT1 or HRVT2 in terms of HR or V\u0307O<jats:sub>2<\/jats:sub>.<\/jats:p>","DOI":"10.14814\/phy2.15782","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:29:35Z","timestamp":1691454575000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effect of ramp slope on intensity thresholds based on correlation properties of heart rate variability during cycling"],"prefix":"10.14814","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3231-3445","authenticated-orcid":false,"given":"Pablo R.","family":"Fleitas\u2010Paniagua","sequence":"first","affiliation":[{"name":"Faculty of Kinesiology University of Calgary  Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0346-7007","authenticated-orcid":false,"given":"Rafael","family":"de Almeida\u00a0Azevedo","sequence":"additional","affiliation":[{"name":"Applied Physiology and Nutrition Research Group \u2010 Center of Lifestyle Medicine, Faculdade de Medicina FMUSP Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mackenzie","family":"Trpcic","sequence":"additional","affiliation":[{"name":"Faculty of Kinesiology University of Calgary  Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6460-6453","authenticated-orcid":false,"given":"Juan M.","family":"Murias","sequence":"additional","affiliation":[{"name":"Faculty of Kinesiology University of Calgary  Calgary Canada"},{"name":"College of Health and Life Sciences Hamad Bin Khalifa University  Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8458-4709","authenticated-orcid":false,"given":"Bruce","family":"Rogers","sequence":"additional","affiliation":[{"name":"College of Medicine University of Central Florida  Orlando Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.611550"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1986.60.6.2020"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3968393"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00942.2016"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/096228029900800204"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100043\u2010110680"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2165\/11599690\u2010000000000\u201000000"},{"key":"e_1_2_9_9_1","first-page":"614","article-title":"Biostatistics 104: Correlational analysis","volume":"44","author":"Chan Y. 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