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However, CWI impacts muscle adaptations to exercise training, with attenuated muscle hypertrophy and increased angiogenesis. Tissue temperature modulates the abundance of specific miRNA species and thus CWI may affect muscle adaptations via modulating miRNA expression following a bout of exercise. The current study focused on the regulatory mechanisms involved in cleavage and nuclear export of mature miRNA, including DROSHA<\/jats:italic>, EXPORTIN\u20105<\/jats:italic>, and DICER<\/jats:italic>. Muscle biopsies were obtained from the vastus lateralis<\/jats:italic> of young males (n<\/jats:italic>\u2009=\u20099) at rest and at 2, 4, and 48\u2009h of recovery from an acute bout of resistance exercise, followed by either 10\u2009min of active recovery (ACT) at ambient temperature or CWI at 10\u00b0C. The abundance of key miRNA species in the regulation of intracellular anabolic signaling (miR\u20101 and miR\u2010133a) and angiogenesis (miR\u201015a and miR\u2010126) were measured, along with several gene targets implicated in satellite cell dynamics (NCAM and PAX7) and angiogenesis (VEGF and SPRED\u20101). When compared to ACT, CWI suppressed mRNA expression of DROSHA<\/jats:italic> (24\u2009h p<\/jats:italic>\u2009=\u20090.025 and 48\u2009h p<\/jats:italic>\u2009=\u20090.017), EXPORTIN\u20105<\/jats:italic> (24\u2009h p<\/jats:italic>\u2009=\u20090.008), and DICER<\/jats:italic> (24\u2009h p<\/jats:italic>\u2009=\u20090.0034). Of the analyzed miRNA species, miR\u2010133a (24\u2009h p<\/jats:italic>\u2009<\u20090.001 and 48\u2009h p<\/jats:italic>\u2009=\u20090.007) and miR\u2010126 (24\u2009h p<\/jats:italic>\u2009<\u20090.001 and 48\u2009h p<\/jats:italic>\u2009<\u20090.001) remained elevated at 24\u2009h post\u2010exercise in the CWI trial only. Potential gene targets of these miRNA, however, did not differ between trials. CWI may therefore impact miRNA abundance in skeletal muscle, although the precise physiological relevance needs further investigation.<\/jats:p>","DOI":"10.14814\/phy2.15784","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:29:16Z","timestamp":1691454556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cold water immersion in recovery following a single bout resistance exercise suppresses mechanisms of miRNA<\/scp> nuclear export and maturation"],"prefix":"10.14814","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4467-1709","authenticated-orcid":false,"given":"Randall F.","family":"D'Souza","sequence":"first","affiliation":[{"name":"Liggins Institute The University of Auckland Auckland New Zealand"},{"name":"Discipline of Nutrition The University of Auckland Auckland New Zealand"},{"name":"Maurice Wilkins Centre for Molecular Biodiscovery The University of 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