{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:12:30Z","timestamp":1753884750280,"version":"3.41.2"},"reference-count":49,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cardiac ischemic reperfusion injury (IRI) is paradoxically instigated by reestablishing blood\u2010flow to ischemic myocardium typically from a myocardial infarction (MI). Although revascularization following MI remains the standard of care, effective strategies remain limited to prevent or attenuate IRI. We hypothesized that epicardial placement of human placental amnion\/chorion (HPAC) grafts will protect against IRI. Using a clinically relevant model of IRI, swine were subjected to 45\u2009min percutaneous ischemia followed with (MI\u2009+\u2009HPAC, <jats:italic>n<\/jats:italic>\u2009=\u20093) or without (MI only, <jats:italic>n<\/jats:italic>\u2009=\u20093) HPAC. Cardiac function was assessed by echocardiography, and regional punch biopsies were collected 14\u2009days post\u2010operatively. A deep phenotyping approach was implemented by using histological interrogation and incorporating global proteomics and transcriptomics in nonischemic, ischemic, and border zone biopsies. Our results established HPAC limited the extent of cardiac injury by 50% (11.0\u2009\u00b1\u20092.0% vs. 22.0\u2009\u00b1\u20093.0%, <jats:italic>p<\/jats:italic>\u2009=\u20090.039) and preserved ejection fraction in HPAC\u2010treated swine (46.8\u2009\u00b1\u20092.7% vs. 35.8\u2009\u00b1\u20094.5%, <jats:italic>p<\/jats:italic>\u2009=\u20090.014). We present comprehensive transcriptome and proteome profiles of infarct (IZ), border (BZ), and remote (RZ) zone punch biopsies from swine myocardium during the proliferative cardiac repair phase 14\u2009days post\u2010MI. Both HPAC\u2010treated and untreated tissues showed regional dynamic responses, whereas only HPAC\u2010treated IZ revealed active immune and extracellular matrix remodeling. Decreased endoplasmic reticulum (ER)\u2010dependent protein secretion and increased antiapoptotic and anti\u2010inflammatory responses were measured in HPAC\u2010treated biopsies. We provide quantitative evidence HPAC reduced cardiac injury from MI in a preclinical swine model, establishing a potential new therapeutic strategy for IRI. Minimizing the impact of MI remains a central clinical challenge. We present a new strategy to attenuate post\u2010MI cardiac injury using HPAC in a swine model of IRI. Placement of HPAC membrane on the heart following MI minimizes ischemic damage, preserves cardiac function, and promotes anti\u2010inflammatory signaling pathways.<\/jats:p>","DOI":"10.14814\/phy2.15838","type":"journal-article","created":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T04:19:56Z","timestamp":1697602796000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Epicardial placement of human placental membrane protects from heart injury in a swine model of myocardial infarction"],"prefix":"10.14814","volume":"11","author":[{"given":"Rinku S.","family":"Skaria","sequence":"first","affiliation":[{"name":"Department of Physiology University of Arizona College of Medicine  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marissa A.","family":"Lopez\u2010Pier","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Arizona College of Engineering  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brij S.","family":"Kathuria","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Arizona College of Medicine  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian J.","family":"Leber","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Arizona College of Medicine  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R.","family":"Langlais","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Arizona College of Medicine  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shravan G.","family":"Aras","sequence":"additional","affiliation":[{"name":"Center for Biomedical and Informatics University of Arizona Health Sciences  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zain I.","family":"Khalpey","sequence":"additional","affiliation":[{"name":"Northwest Healthcare  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela G.","family":"Hitscherich","sequence":"additional","affiliation":[{"name":"MTF Biologics  Edison New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evangelia","family":"Chnari","sequence":"additional","affiliation":[{"name":"MTF Biologics  Edison New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Long","sequence":"additional","affiliation":[{"name":"MTF Biologics  Edison New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jared M.","family":"Churko","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine University of Arizona College of Medicine  Tucson Arizona USA"},{"name":"Sarver Molecular Cardiovascular Research Program University of Arizona College of Medicine  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond B.","family":"Runyan","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine University of Arizona College of Medicine  Tucson Arizona USA"},{"name":"Sarver Molecular Cardiovascular Research Program University of Arizona College of Medicine  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3579-087X","authenticated-orcid":false,"given":"John P.","family":"Konhilas","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Arizona College of Medicine  Tucson Arizona USA"},{"name":"Department of Biomedical Engineering University of Arizona College of Engineering  Tucson Arizona USA"},{"name":"Sarver Molecular Cardiovascular Research Program University of Arizona College of Medicine  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970020213"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/295325a0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd7010001"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)91212-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2019.21326"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAT.0000000000000727"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.11.068"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/7\/4\/045011"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2013.01.004"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1974.01360030022006"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)19017-5"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-009-9144-1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12954"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2021.112512"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.87.3.755"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt171"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI62874"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2014.08.028"},{"issue":"2","key":"e_1_2_9_20_1","first-page":"100","article-title":"Correlation of early and late ejection fractions with CCL5 and CCL18 levels in acute anterior myocardial infarction","volume":"13","author":"Khanian M. S.","year":"2016","journal-title":"Iranian Journal of Immunology"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060519857839"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13019\u2010017\u20100567\u20107"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1536\/ihj.52.382"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehr127"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070885"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7018393"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v015a07"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198603273141303"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.08.089"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-198610000-00016"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2016.0530"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2018.01.83"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-012-9337-x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-018-0197-1"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0000000000009650"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0007-1226(95)90123-X"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66327-9"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1159\/000303050"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-011-0232-6"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(79)90957-8"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000001123"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3901"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvr113"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.2217\/fca-2021-0017"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00291"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075383"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.76.1.44"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.942250"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2013.04.023"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.12.021"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.15838","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T04:44:16Z","timestamp":1703565856000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.15838"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10]]},"references-count":49,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.14814\/phy2.15838"],"URL":"https:\/\/doi.org\/10.14814\/phy2.15838","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"type":"print","value":"2051-817X"},{"type":"electronic","value":"2051-817X"}],"subject":[],"published":{"date-parts":[[2023,10]]},"assertion":[{"value":"2023-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e15838"}}