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Cooling increases communication through myoendothelial gap junctions (MEGJs), thereby increasing endothelium\u2010derived hyperpolarization (EDH)\u2010type dilatation. EDH is initiated by calcium\u2010activated potassium channels (K<jats:sub>Ca<\/jats:sub>) activated by endothelial stimuli or muscle\u2010derived mediators traversing MEGJs (myoendothelial feedback). The goal of this study was to determine the individual roles of K<jats:sub>Ca<\/jats:sub> with small (SK3) and intermediate (IK1) conductance in cooling\u2010induced dilatation. Vasomotor responses of mice isolated cutaneous tail arteries were analyzed by pressure myography at 37\u00b0C and 28\u00b0C. Cooling increased acetylcholine\u2010induced EDH\u2010type dilatation during inhibition of NO and prostacyclin production. IK1 inhibition did not affect dilatations to acetylcholine, whereas SK3 inhibition inhibited dilatation at both temperatures. Cooling uncovered myoendothelial feedback to inhibit constrictions in U46619. IK1 inhibition did not affect U46619 constrictions, whereas SK3 inhibition abolished the inhibitory effect of cooling without affecting U46619 constriction at 37\u00b0C. Immunoblots confirmed SK3 expression, which was localized (immunofluorescence) to holes in the internal elastic lamina consistent with myoendothelial projections. Immunoblots and Immunofluorescence did not detect IK1. Studies in non\u2010cutaneous arteries have highlighted the predominant role of IK1 in EDH\u2010type dilatation. Cutaneous arteries are distinctly reliant on SK3, which may enable EDH\u2010type dilation to be amplified by cooling.<\/jats:p>","DOI":"10.14814\/phy2.15884","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:48:13Z","timestamp":1701132493000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cooling\u2010induced cutaneous vasodilatation is mediated by small\u2010conductance, calcium\u2010activated potassium channels in tail arteries from male mice"],"prefix":"10.14814","volume":"11","author":[{"given":"Fumin","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheila","family":"Flavahan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0840-0298","authenticated-orcid":false,"given":"Nicholas A.","family":"Flavahan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000128407.45014.58"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01305.2004"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.110.165795"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.278.4.H1075"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00268.2003"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705756"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6529"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.172379"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00306.2007"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.076653"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.195"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1526-2_4"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1985.249.5.H950"},{"key":"e_1_2_10_15_1","first-page":"589","article-title":"Denervation augments alpha\u20102 but not alpha\u20101 adrenergic responses in canine saphenous veins","volume":"240","author":"Flavahan N. 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