{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T02:06:29Z","timestamp":1776132389310,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007880","name":"Johns Hopkins University","doi-asserted-by":"publisher","award":["StAAR award x2"],"award-info":[{"award-number":["StAAR award x2"]}],"id":[{"id":"10.13039\/100007880","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Wire myography to test vasomotor functions of blood vessels ex\u2010vivo are well\u2010established for the systemic circulation, however, there is no consensus on protocols for pulmonary arteries. We created a standardized wire myography protocol for healthy rat PAs and validated this in a pulmonary hypertension (PH) model. Vessels stretched to higher initial tensions (5.0, 7.5 and 10.0\u2009mN) exhibited a uniform response to phenylephrine, a larger dynamic range, and lower EC50 values. The endothelium\u2010mediated relaxation showed that moderate tensions (7.5 and 10.0\u2009mN) produced robust responses with higher maximum relaxation and lower EC50 values. For endothelium independent responses, the higher initial tension groups had lower and more consistent EC50 values than the lower initial tension groups. Pulmonary arteries from rats with PH were more responsive to vasoactive drugs when subjected to a higher initial tension. Notably, vessels in the PH group subjected to 15.0\u2009mN exhibited high dynamic ranges in contractile and relaxation responses without tearing. Lastly, we observed attenuated cholinergic responses in these vessels\u2014consistent with endothelial dysfunction in PH. Therefore, a moderate initial tension of 7.5\u201310.0\u2009mN is optimal for healthy rat pulmonary arteries and a higher initial tension of 15.0\u2009mN is optimal for pulmonary arteries from animals with PH.<\/jats:p>","DOI":"10.14814\/phy2.15911","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T03:55:46Z","timestamp":1705031746000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Optimization of resting tension for wire myography in male rat pulmonary arteries"],"prefix":"10.14814","volume":"12","author":[{"given":"Rira","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Johns Hopkins University  Baltimore Maryland USA"},{"name":"Department of Anesthesiology and Critical Care Medicine Johns Hopkins School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roshini","family":"Narayanan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep","family":"Jandu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine Johns Hopkins School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Savage","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine Johns Hopkins School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bulouere","family":"Wodu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine Johns Hopkins School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kavitha","family":"Nandakumar","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine Johns Hopkins School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshmi","family":"Santhanam","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Johns Hopkins University  Baltimore Maryland USA"},{"name":"Department of Anesthesiology and Critical Care Medicine Johns Hopkins School of Medicine  Baltimore Maryland USA"},{"name":"Department of Biomedical Engineering Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9856-512X","authenticated-orcid":false,"given":"Jochen","family":"Steppan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine Johns Hopkins School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2016-0418OC"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/13.Supplement_6.S454"},{"key":"e_1_2_10_4_1","article-title":"Mesenteric artery contraction and relaxation studies using automated wire myography","volume":"55","author":"Bridges L. 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