{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:14:27Z","timestamp":1753884867214,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As the molecular mechanism of nephrotic syndrome remains largely undiscovered, patients continue to be exposed to the pros and cons of uniform glucocorticoid treatment. We explored whether the exposure of in\u00a0vitro\u2010cultivated podocytes to sera from children with steroid\u2010sensitive or steroid\u2010resistant nephrotic syndrome induces differences in gene expression profiles, which could help to elucidate the pathogenesis of the steroid response. Human immortalized podocytes were cultivated with patient sera for 3\u2009days. After cell lysis, RNA extraction, 3\u2032\u2010mRNA libraries were prepared and sequenced. There were 34 significantly upregulated and 14 downregulated genes (fold difference &lt;0.5 and &gt;2.0, respectively, and false discovery rate\u2010corrected <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and 22 significantly upregulated and 6 downregulated pathways (false discovery rate\u2010corrected <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) in the steroid\u2010sensitive (<jats:italic>n<\/jats:italic>\u2009=\u20099) versus steroid\u2010resistant group (<jats:italic>n<\/jats:italic>\u2009=\u20094). The observed pathways included upregulated redox reactions, DNA repair, mitosis, protein translation and downregulated cholesterol biosynthesis. Sera from children with nephrotic syndrome induce disease subtype\u2010specific transcriptome changes in human podocytes in\u00a0vitro. However, further exploration of a larger cohort is needed to verify whether clinically distinct types of nephrotic syndrome or disease activity may be differentiated by specific transcriptomic profiles and whether this information may help to elucidate the pathogenesis of the steroid response.<\/jats:p>","DOI":"10.14814\/phy2.15932","type":"journal-article","created":{"date-parts":[[2024,2,3]],"date-time":"2024-02-03T02:13:25Z","timestamp":1706926405000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nephrotic syndrome sera induce different transcriptomes in podocytes based on the steroid response"],"prefix":"10.14814","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6961-5853","authenticated-orcid":false,"given":"Martin","family":"Bezdicka","sequence":"first","affiliation":[{"name":"Vera Vavrova Lab\/VIAL, Department of Pediatrics, Second Faculty of Medicine Charles University and Motol University 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