{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:00:43Z","timestamp":1771261243404,"version":"3.50.1"},"reference-count":68,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T00:00:00Z","timestamp":1707091200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH R01 HL141198"],"award-info":[{"award-number":["NIH R01 HL141198"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH R01 HL164571"],"award-info":[{"award-number":["NIH R01 HL164571"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A feature of peripheral artery diseases (PAD) includes limb ischemia\/reperfusion (I\/R) and ischemia. Both I\/R and ischemia amplify muscle afferent nerve\u2010activated reflex sympathetic nervous and blood pressure responses (termed as exercise pressor reflex). Nevertheless, the underlying mechanisms responsible for the exaggerated autonomic responses in PAD are undetermined. Previous studies suggest that acid\u2010sensing ion channels (ASICs) in muscle dorsal root ganglion (DRG) play a leading role in regulating the exercise pressor reflex in PAD. Thus, we determined if signaling pathways of nerve growth factor (NGF) contribute to the activities of ASICs in muscle DRG neurons of PAD. In particular, we examined ASIC1a and ASIC3 currents in isolectin B<jats:sub>4<\/jats:sub>\u2010negative muscle DRG neurons, a distinct subpopulation depending on NGF for survival. Hindlimb I\/R and ischemia were obtained in male rats. In results, femoral artery occlusion increased the levels of NGF and NGF\u2010stimulated TrkA receptor in DRGs, whereas they led to upregulation of ASIC3 but not ASIC1a. In addition, application of NGF onto DRG neurons increased the density of ASIC3 currents and the effect of NGF was significantly attenuated by TrkA antagonist GW441756. Moreover, the enhancing effect of NGF on the density of ASIC3\u2010like currents was decreased by the respective inhibition of intracellular signaling pathways, namely JNK and NF\u2010\u03baB, by antagonists SP600125 and PDTC. Our results suggest contribution of NGF to the activities of ASIC3 currents via JNK and NF\u2010\u03baB signaling pathways in association with the exercise pressor reflex in experimental PAD.<\/jats:p>","DOI":"10.14814\/phy2.15933","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T08:35:22Z","timestamp":1707294922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>NGF<\/scp> contributes to activities of acid\u2010sensing ion channels in dorsal root ganglion neurons of male rats with experimental peripheral artery disease"],"prefix":"10.14814","volume":"12","author":[{"given":"Qin","family":"Li","sequence":"first","affiliation":[{"name":"Heart and Vascular Institute The Pennsylvania State University College of Medicine  Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0513-3983","authenticated-orcid":false,"given":"Jianhua","family":"Li","sequence":"additional","affiliation":[{"name":"Heart and Vascular Institute The Pennsylvania State University College of Medicine  Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2018.03.008"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460\u20109568.1995.tb01143.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/000331979905000502"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1475\u2010097X.2007.00720.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2023.1111170"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8295054"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0969\u20102126(97)00280\u20103"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.18\u201008\u201003059.1998"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00401.2019"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1971.sp009498"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.116.303849"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2008.05.032"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5266-10.2011"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.213"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00371.2002"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00257.2020"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00565.2019"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jp275058"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2016.179"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1253\/circj.CJ\u201016\u20101286"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00641.2017"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.129197"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00623.2008"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn0901\u2010869"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.cp120110"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10286\u2010002\u20100059\u20101"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00137.2020"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00338.2019"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1113\/jp281704"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14935"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15654"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1213\/ane.0b013e318168514b"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00612.2010"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.05.041"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.278.3.R563"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.85.4.1583"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.22\u201024\u201010662.2002"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309468200"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12199"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.17\u201021\u201008476.1997"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.45.030183.001305"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0896\u20106273(00)80966\u20106"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.241281"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1134\/s0006297914130069"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(01)06351\u20106"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0002\u20108703(99)70361\u20103"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.113035"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc4401.098491"},{"key":"e_1_2_11_50_1","article-title":"Exaggerated blood pressure response to static exercise in hindlimb ischemia\u2010reperfusion","volume":"13","author":"Qin L.","year":"2022","journal-title":"Frontiers in Exercise Physiol"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1910905116"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e31821ecf61"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2014.09.003"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1989.67.1.256"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00188.2012"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020374"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01405.2004"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00630.2015"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2014.10.014"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10286\u2010022\u201000872\u20103"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0209250"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.217851"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2016.06.099"},{"key":"e_1_2_11_64_1","first-page":"333","volume-title":"Handbook of physiology","author":"Waldrop T. G.","year":"1996"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00107.2004"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106340"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2019.08.017"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.221788"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000238388.79295.4c"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.15933","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T14:53:10Z","timestamp":1708095190000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.15933"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":68,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.14814\/phy2.15933"],"URL":"https:\/\/doi.org\/10.14814\/phy2.15933","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"value":"2051-817X","type":"print"},{"value":"2051-817X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-11-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e15933"}}