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We tested whether intralaryngeal (local) application of FNT would also trigger an apnea and whether the apneic response to both local and systemic administration of FNT was laryngeal afferent\u2010mediated. Cardiorespiratory responses to FNT were recorded in anesthetized male adult rats with and without bilateral sectioning of the superior laryngeal nerve (SLNx) or peri\u2010SLN capsaicin treatment (SLNcap) to block local C\u2010fiber signal conduction. Opioid mu\u2010receptor (MOR)\u2010immunoreactivity was detected in laryngeal C\u2010 and myelinated neurons. We found that local and systemic administration of FNT elicited an immediate apnea. SLNx, rather than SLNcap, abolished the apneic response to local FNT application though MORs were abundantly expressed in both laryngeal C\u2010 and myelinated neurons. Importantly, SLNx failed to affect the apneic response to systemic FNT administration. These results lead to the conclusion that laryngeal afferents' MORs are responsible for the apneic response to local, but not systemic, administration of FNT.<\/jats:p>","DOI":"10.14814\/phy2.15965","type":"journal-article","created":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T04:40:27Z","timestamp":1709700027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Apneic response to fentanyl in adult rats: Role of laryngeal afferents"],"prefix":"10.14814","volume":"12","author":[{"given":"Jianguo","family":"Zhuang","sequence":"first","affiliation":[{"name":"Department of Physiology Lovelace Biomedical Research Institute  Albuquerque New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuping","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Physiology Lovelace Biomedical Research Institute  Albuquerque New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Physiology Lovelace Biomedical Research Institute  Albuquerque New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2345-3785","authenticated-orcid":false,"given":"Fadi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Physiology Lovelace Biomedical Research Institute  Albuquerque New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2001.90.4.1570"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348949310201007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/pmj.77.908.371"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1982.53.2.455"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2006.10.005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1974.sp010605"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563650.2016.1157722"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00105.2011"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90568-9"},{"key":"e_1_2_9_11_1","article-title":"The fiber composition of the superior laryngeal nerve","volume":"7","author":"Chung K. 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