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We aimed to explore the effects of cholecalciferol (VD<jats:sub>3<\/jats:sub>) and calcitriol (1,25VD<jats:sub>3<\/jats:sub>) on signaling of muscle proteolysis and oxidative stress in myotubes challenged with angiotensin II (AII). The mouse C2C12 myotubes were assigned to vehicle, AII, AII\u2009+\u2009VD<jats:sub>3<\/jats:sub>, AII\u2009+\u20091,25VD<jats:sub>3<\/jats:sub>, and AII\u2009+\u2009losartan groups. The expression levels of muscle\u2010specific E3 ubiquitin ligase proteins, autophagy\u2010related proteins, and oxidative stress markers were investigated. We demonstrated the diverse effects of VD<jats:sub>3<\/jats:sub> and 1,25VD<jats:sub>3<\/jats:sub> on AII\u2010induced myotube atrophy. The myotube diameter was preserved by treatment with 100\u2009nM VD<jats:sub>3<\/jats:sub> and losartan, while 1 and 10\u2009nM 1,25VD<jats:sub>3<\/jats:sub> increased levels of FoxO3a, MuRF1, and atrogin\u20101 protein expression in myotubes exposed to AII. Treatment with AII\u2009+\u200910\u2009nM 1,25VD<jats:sub>3<\/jats:sub> resulted in the upregulation of LC3B\u2010II, LC3B\u2010II\/LC3B\u2010I, and mature cathepsin L, which are autophagic marker proteins. The p62\/SQSTM1 protein was downregulated and vitamin D receptor was upregulated after treatment with AII\u2009+\u200910\u2009nM 1,25VD<jats:sub>3<\/jats:sub>. A cellular redox imbalance was observed as AII\u2009+\u200910\u2009nM 1,25VD<jats:sub>3<\/jats:sub>\u2010induced reactive oxygen species and NADPH oxidase\u20102 overproduction, and these changes were associated with an inadequate response of antioxidant superoxide dismutase\u20101 and catalase proteins. Collectively, these findings provide a translational perspective on the role of vitamin D<jats:sub>3<\/jats:sub> in alleviating muscle atrophy related to high levels of AII.<\/jats:p>","DOI":"10.14814\/phy2.16011","type":"journal-article","created":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T02:24:43Z","timestamp":1713320683000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Differential effects of cholecalciferol and calcitriol on muscle proteolysis and oxidative stress in angiotensin <scp>II<\/scp>\u2010induced <scp>C2C12<\/scp> myotube atrophy"],"prefix":"10.14814","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2998-9353","authenticated-orcid":false,"given":"Muthita","family":"Hirunsai","sequence":"first","affiliation":[{"name":"Department of Biopharmacy, Faculty of Pharmacy Srinakharinwirot University  Nakhon Nayok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6583-7667","authenticated-orcid":false,"given":"Ratchakrit","family":"Srikuea","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Science Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0471-7_2"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0000000000001150"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjms.2016.04.016"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00180-10"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00204.2014"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.4.8082"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP281211"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.09.009"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200901052"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00403.2011"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2012-1012"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1016"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2021.104883"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119352"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.13746"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3791\/60682"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2245"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004973"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215219"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2014.07.017"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2009.28113"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22115417"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085207"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00192.2009"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.05.205"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2015-1685"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)08024-8"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0b013e328352b4c2"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2007.04.003"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-12-0234"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00400-3"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-110163"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2017.01.040"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00898.2018"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01018.2015"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13269"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202669"},{"key":"e_1_2_11_39_1","first-page":"63","article-title":"Angiotensin receptor blocker\u2010induced visceral angioedema","volume":"25","author":"Thalanayar M. 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