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Candidates include the OSCA\/TMEM63 family of ion channels, which can function as mechanosensors and osmosensors. Using <jats:italic>Tmem63b<\/jats:italic><jats:sup><jats:italic>HA\u2010fl\/HA\u2010fl<\/jats:italic><\/jats:sup> reporter mice, we assessed the localization of HA\u2010tagged\u2010TMEM63B within the urinary tract by immunofluorescence coupled with confocal microscopy. In the kidneys, HA\u2010TMEM63B was expressed by proximal tubule epithelial cells, by the intercalated cells of the collecting duct, and by the epithelial cells lining the thick ascending limb of the medulla. In the urinary tract, HA\u2010TMEM63B was expressed by the urothelium lining the renal pelvis, ureters, bladder, and urethra. HA\u2010TMEM63B was also expressed in closely allied organs including the epithelial cells lining the seminal vesicles, vas deferens, and lateral prostate glands of male mice and the vaginal epithelium of female mice. Our studies reveal that TMEM63B is expressed by subsets of kidney and lower urinary tract epithelial cells, which we hypothesize are sites of TMEM63B mechanosensation or osmosensation, or both.<\/jats:p>","DOI":"10.14814\/phy2.16043","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T03:21:21Z","timestamp":1715311281000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Expression and localization of the mechanosensitive\/osmosensitive ion channel <scp>TMEM63B<\/scp> in the mouse urinary tract"],"prefix":"10.14814","volume":"12","author":[{"given":"Marianela G.","family":"Dalghi","sequence":"first","affiliation":[{"name":"Department of Medicine and George M. 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