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Male MK5 haplodeficient mice show reduced hypertrophy and attenuated increase in\n                    <jats:italic>Col1a1<\/jats:italic>\n                    mRNA in response to increased cardiac afterload. In addition, MK5 deficiency impairs cardiac fibroblast function. This study determined the effect of reduced ERK3 on cardiac hypertrophy following transverse aortic constriction (TAC) and fibroblast biology in male mice. Three weeks post\u2010surgery, ERK3, but not ERK4 or p38\u03b1, co\u2010immunoprecipitated with MK5 from both sham and TAC heart lysates. The increase in left ventricular mass and myocyte diameter was lower in TAC\u2010ERK3\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    than TAC\u2010ERK3\n                    <jats:sup>+\/+<\/jats:sup>\n                    hearts, whereas ERK3 haploinsufficiency did not alter systolic or diastolic function. Furthermore, the TAC\u2010induced increase in\n                    <jats:italic>Col1a1<\/jats:italic>\n                    mRNA abundance was diminished in ERK3\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    hearts. ERK3 immunoreactivity was detected in atrial and ventricular fibroblasts but not myocytes. In both quiescent fibroblasts and \u201cactivated\u201d myofibroblasts isolated from adult mouse heart, siRNA\u2010mediated knockdown of ERK3 reduced the TGF\u2010\u03b2\u2010induced increase in\n                    <jats:italic>Col1a1<\/jats:italic>\n                    mRNA. In addition, intracellular type 1 collagen immunoreactivity was reduced following ERK3 depletion in quiescent fibroblasts but not myofibroblasts. Finally, knocking down ERK3 impaired motility in both atrial and ventricular myofibroblasts. These results suggest that ERK3 plays an important role in multiple aspects of cardiac fibroblast biology.\n                  <\/jats:p>","DOI":"10.14814\/phy2.16108","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T03:29:03Z","timestamp":1718335743000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>ERK3<\/scp>\n                    is involved in regulating cardiac fibroblast function"],"prefix":"10.14814","volume":"12","author":[{"given":"Pramod","family":"Sahadevan","sequence":"first","affiliation":[{"name":"Montreal Heart Institute  Montr\u00e9al Qu\u00e9bec Canada"},{"name":"Department of Biochemistry and Molecular Medicine Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dharmendra","family":"Dingar","sequence":"additional","affiliation":[{"name":"Montreal Heart Institute  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