{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T14:44:18Z","timestamp":1782312258384,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL143452"],"award-info":[{"award-number":["R01HL143452"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Skeletal muscle dysfunction in critical illnesses leaves survivors weak and functionally impaired. Macrophages infiltrate muscles; however, their functional role is unclear. We aim to examine muscle leukocyte composition and the effect of macrophages on muscle mass and function in the murine acute lung injury (ALI)\u2010associated skeletal muscle wasting model. We performed flow cytometry of hindlimb muscle to identify myeloid cells pre\u2010injury and time points up to 29\u2009days after intratracheal lipopolysaccharide ALI. We evaluated muscle force and morphometrics after systemic and intramuscular clodronate\u2010induced macrophage depletions between peak lung injury and recovery (day 5\u20136) versus vehicle control. Our results show muscle leukocytes changed over ALI course with day 3 neutrophil infiltration (130.5\u2009\u00b1\u200995.6cells\/mg control to 236.3\u2009\u00b1\u200970.6cells\/mg\u2009day 3) and increased day 10 monocyte abundance (5.0\u2009\u00b1\u20093.4%CD45<jats:sup>+<\/jats:sup>CD11b<jats:sup>+<\/jats:sup> day 3 to 14.0\u2009\u00b1\u20092.6%CD45<jats:sup>+<\/jats:sup>CD11b<jats:sup>+<\/jats:sup> day 10, <jats:italic>p<\/jats:italic>\u2009=\u20090.005). Although macrophage count did not significantly change, pro\u2010inflammatory (27.0\u2009\u00b1\u20097.2% day 3 to 7.2\u2009\u00b1\u20093.8% day 10, <jats:italic>p<\/jats:italic>\u2009=\u20090.02) and anti\u2010inflammatory (30.5\u2009\u00b1\u200911.1% day 3 to 52.7\u2009\u00b1\u20099.7% day 10, <jats:italic>p<\/jats:italic>\u2009=\u20090.09) surface marker expression changed over the course of ALI. Macrophage depletion following peak lung injury increased muscle mass and force generation. These data suggest muscle macrophages beyond peak lung injury limit or delay muscle recovery. Targeting macrophages could augment muscle recovery following lung injury.<\/jats:p>","DOI":"10.14814\/phy2.70052","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T01:23:58Z","timestamp":1727400238000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Macrophages modulate skeletal muscle wasting and recovery in acute lung injury in mice"],"prefix":"10.14814","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9847-7234","authenticated-orcid":false,"given":"Jennifer T. W.","family":"Krall","sequence":"first","affiliation":[{"name":"Section on Pulmonary, Critical Care, Allergy, and Immunologic Disease, Department of Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanazha","family":"Belfield","sequence":"additional","affiliation":[{"name":"Section on Pulmonary, Critical Care, Allergy, and Immunologic Disease, Department of Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Strysick","sequence":"additional","affiliation":[{"name":"Section on Pulmonary, Critical Care, Allergy, and Immunologic Disease, Department of Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Liu","sequence":"additional","affiliation":[{"name":"Section on Pulmonary, Critical Care, Allergy, and Immunologic Disease, Department of Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Purcell","sequence":"additional","affiliation":[{"name":"Section on Pulmonary, Critical Care, Allergy, and Immunologic Disease, Department of Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renee","family":"Stapleton","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Medicine University of Vermont College of Medicine  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Toth","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Medicine University of Vermont College of Medicine  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Poynter","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Medicine University of Vermont College of Medicine  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuewei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Section on Molecular Medicine, Department of Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Gibbs","sequence":"additional","affiliation":[{"name":"Section on Pulmonary, Critical Care, Allergy, and Immunologic Disease, Department of Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Clark","family":"Files","sequence":"additional","affiliation":[{"name":"Section on Pulmonary, Critical Care, Allergy, and Immunologic Disease, Department of Medicine Wake Forest University School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070075"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-020-00266-1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017279"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20220525"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00419.2015"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36498"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000002208"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00201.2007"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000000040"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-022-04253-0"},{"issue":"923","key":"e_1_2_8_12_1","first-page":"2023","article-title":"Global deletion of CCL2 has adverse impacts on recovery of skeletal muscle fiber size and function and is muscle specific","volume":"134","author":"Ferrara P. 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