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This study aimed to assess particle size, concentration and microRNA (miR) content within small EV\u2010enriched separations prepared from human blood taken before and after unaccustomed eccentric\u2010biased exercise\u2010induced muscle damage. Nine male volunteers underwent plyometric jumping and downhill running, with blood samples taken at baseline, 2, and 24\u2009h post\u2010exercise. EVs were separated using size exclusion chromatography (SEC) and their characteristics evaluated by nanoparticle tracking. No changes in EV size or concentration were seen following the muscle\u2010damaging exercise. Small RNA sequencing identified 240 miRs to be consistently present within the EVs. RT\u2010qPCR analysis was performed: specifically, for known muscle\u2010enriched\/important miRs, including miR\u20101, \u2212206, \u2212133a, \u2212133b, \u221231, \u2212208b, \u2212451a, \u2212486 and\u2009\u2212\u2009499 and the immune\u2010important miR\u201021, \u2212146a and\u2009\u2212\u2009155. Notably, none of the immune\u2010important miRs within the EVs tested changed in response to the muscle damage. Of the muscle\u2010associated miRs tested, only the levels of miR\u201031\u20105p were seen to change with decreased levels at 24\u2009h compared to baseline and 2\u2009h, indicating involvement in the damage response. These findings shed light on the dynamic role of EV miRs in response to exercise\u2010induced muscle damage.<\/jats:p>","DOI":"10.14814\/phy2.70056","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T14:24:10Z","timestamp":1727447050000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of plasma\u2010derived small extracellular vesicle characteristics and <scp>microRNA<\/scp> cargo following exercise\u2010induced skeletal muscle damage in men"],"prefix":"10.14814","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6283-5594","authenticated-orcid":false,"given":"Jason","family":"Lovett","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences Stellenbosch University  Stellenbosch South 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