{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:12:00Z","timestamp":1789564320721,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T00:00:00Z","timestamp":1729296000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL151536"],"award-info":[{"award-number":["R01HL151536"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sirtuin 3 has been shown to regulate endothelial function and coronary flow reserve in mice. Knockout of SIRT3 reduced endothelial nitric oxide synthase expression in the mouse hearts. In this study, we investigate whether endothelial SIRT3 regulates vascular function and myogenic responses in distal intramural branches of the left anterior descending coronary artery (CA) and middle cerebral artery (MCA) of mice. Both male and female endothelial SIRT3 knockout (SIRT3\n                    <jats:sup>EC<\/jats:sup>\n                    KO) mice and control SIRT3\n                    <jats:sup>LoxP<\/jats:sup>\n                    mice were used and CA and MCA were dissected and mounted in a myograph system. The myogenic response was evaluated by measuring changes in inner diameter in response to 20\u2009mmHg stepwise increases in intraluminal pressure in PSS (active diameter) and Ca2\n                    <jats:sup>+<\/jats:sup>\n                    \u2010free PSS (passive diameter). Acetylcholine (Ach)\u2010induced endothelial\u2010dependent relaxation (EDR) and sodium nitroprusside (SNP)\u2010induced endothelial\u2010independent relaxation (EIR) were examined. Our results showed that the myogenic responses were significantly impaired in both the CA and MCA of SIRT3\n                    <jats:sup>EC<\/jats:sup>\n                    KO mice. Furthermore, female mice had worsened myogenic response in MCA. In CA, EDR was abolished in both male and female SIRT3\n                    <jats:sup>EC<\/jats:sup>\n                    KO mice. Intriguingly, EIR was only reduced in the female mice. In MCA, EDR was reduced in male SIRT3\n                    <jats:sup>EC<\/jats:sup>\n                    KO mice, whereas EIR was decreased in both male and female mice. Female SIRT3\n                    <jats:sup>EC<\/jats:sup>\n                    KO mice had profound dysfunction in CA, whereas male mice exhibited more dysfunction in MCA. These data revealed a sex and organ\u2010specific role of endothelial SIRT3 in vascular function and myogenic responses. Our study suggests that endothelial SIRT3 is necessary for maintaining vascular function and blood flow autoregulation.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70060","type":"journal-article","created":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T03:38:49Z","timestamp":1729309129000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Knockout of Sirtuin 3 in endothelial cells impairs endothelial\u2010dependent relaxation and myogenic response in mice"],"prefix":"10.14814","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9203-4336","authenticated-orcid":false,"given":"Jian\u2010Xiong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, School of Medicine University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, School of Medicine University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, School of Medicine University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8559-2807","authenticated-orcid":false,"given":"Heng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, School of Medicine University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000001354"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.046904"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097737"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000020712.84444.8D"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2022.101813"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.121.022070"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00789441"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120030"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqad002"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2016.04.092"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27200"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2017.09.007"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.226464"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00352.2003"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199501000-00002"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/15701611113116660150"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2021.03.293"},{"key":"e_1_2_11_19_1","first-page":"S33","article-title":"The myogenic response in the microcirculation and its interaction with other control systems","volume":"7","author":"Johnson P. 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