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To test the role of <jats:italic>Ift88<\/jats:italic> in T cell development, we performed flow cytometry analysis on thymus and spleen tissue isolated from mice lacking <jats:italic>Ift88<\/jats:italic> in thymic epithelial cells (TECs) or T cells. Analyses indicated that TEC <jats:italic>Ift88<\/jats:italic> deletion had no impact on thymic T cell development and minimal impact on splenic T cells. Analysis of T cells in <jats:italic>CaggCre<\/jats:italic><jats:sup><jats:italic>ERT2+<\/jats:italic><\/jats:sup><jats:italic>Ift88<\/jats:italic> <jats:sup>tm1Bky<\/jats:sup>mTmG mice indicate that approximately half of DN1 thymocytes are <jats:italic>Ift88<\/jats:italic> deficient 3\u2009weeks post\u2010tamoxifen induction; <jats:italic>Ift88<\/jats:italic> loss did not impact T cell development at the DN2\u2010DN4 stage or the CD4+\/CD8+ double\u2010positive (DP) thymocyte stage. However, survival of <jats:italic>Ift88<\/jats:italic> deficient T cells was significantly reduced at the single\u2010positive (SP) thymocyte stage, as was the number of CD4+ and CD8+ T cells in spleen and kidney. Despite preferential survival of <jats:italic>Ift88<\/jats:italic>\u2010proficient cells, the total number of T cells the in spleen and kidney was minimally impacted by <jats:italic>Ift88<\/jats:italic> loss. These data suggest <jats:italic>Ift88<\/jats:italic> is required for differentiation of DP thymocytes into SP thymocytes and that <jats:italic>Ift88<\/jats:italic> proficient T cells can compensate for deficient cells to fill the open niche.<\/jats:p>","DOI":"10.14814\/phy2.70120","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T02:18:26Z","timestamp":1732069106000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["T cell\u2010expressed <i>Ift88<\/i> is required for proper thymocyte differentiation in mice"],"prefix":"10.14814","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4888-5306","authenticated-orcid":false,"given":"Sarah J.","family":"Miller","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology and Hypertension University of Oklahoma Health Sciences Center  Oklahoma City Oklahoma 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Integrative Biology University of Alabama at Birmingham  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1798-8046","authenticated-orcid":false,"given":"Kurt A.","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology and Hypertension University of Oklahoma Health Sciences Center  Oklahoma City Oklahoma 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