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We investigated how cholic acid (CA) supplementation in murine pregnancy impacts adipose tissue function. Mice were fed normal or 0.5% CA\u2010supplemented chow from identification of copulatory plug until gestational day 14 or 15 (<jats:italic>n<\/jats:italic>\u2009=\u200910\u201311\/group) and were matched experimentally with nonpregnant mice (<jats:italic>n<\/jats:italic>\u2009=\u20097\/group). Tissue weights were measured alongside plasma bile acids, glucose, lipids, reactive oxygen metabolites (ROM), and adipokines. Subcutaneous and gonadal adipocyte mRNA expression was evaluated. CA supplementation inhibited pregnancy\u2010associated adipose tissue expansion and decreased fetal weight. CA diet in pregnancy increased LDL\u2010cholesterol and reduced HDL\u2010cholesterol. Pregnancy and CA diet reduced lipid metabolism transcript expression in adipocytes. CA supplementation during pregnancy increased plasma ROM by 1.24\u2010fold and suppressed inflammatory\u2010modulating pentraxin\u20102\/3 and insulin\u2010like growth factor 1 (IGF\u20101) levels by &gt;50% and &gt;80%, respectively. Together, we show that hypercholanemia disturbs pregnancy\u2010associated adipose tissue expansion and mRNA expression in late gestation concomitant with reduced IGF\u20101, altered lipid availability and increased inflammation and oxidation, which could impact fetal growth. This work highlights the need to better understand adipose tissue and redox stress changes in ICP pregnancies and the potential implications for fetal health.<\/jats:p>","DOI":"10.14814\/phy2.70141","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T01:51:01Z","timestamp":1734054661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Gestational hypercholanemia suppresses pregnancy\u2010associated adipose mass increase and stimulates a pro\u2010inflammatory environment in mice"],"prefix":"10.14814","volume":"12","author":[{"given":"Vanya","family":"Nikolova","sequence":"first","affiliation":[{"name":"Department of Women and Children's Health, Guy's Campus King's College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice L.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Women and Children's Health, Guy's Campus King's College London  London UK"},{"name":"Department of Metabolism, Digestion and Reproduction, Hammersmith Campus Imperial College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Bellafante","sequence":"additional","affiliation":[{"name":"Department of Women and Children's Health, Guy's Campus King's College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene","family":"Jansen","sequence":"additional","affiliation":[{"name":"Centre for Health Protection, National Institute for Public Health and the Environment  Bilthoven The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia","family":"Papacleovoulou","sequence":"additional","affiliation":[{"name":"Department of Women and Children's Health, Guy's Campus King's College London  London 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