{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:11:38Z","timestamp":1753884698702,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Facilitative UT\u2010B urea transporters in the brain play an important role in regulating levels of urea in various cell types, including astrocytes. Numerous studies have reported increased UT\u2010B RNA expression with aging and in neurological disorders, such as Alzheimer's Disease. However, much less is known about the effects of these conditions on UT\u2010B transporter protein abundance. This current study compared the levels of UT\u2010B RNA and protein in young and aged male C57BL\/6 mice. Endpoint RT\u2010PCR experiments showed UT\u2010B RNA expression increased in both aged cortex and aged hippocampus. Importantly, these changes were coupled with an increase in protein abundance, as western blotting revealed that 30\u201335\u2009kDa UT\u2010B1 protein was significantly increased in aged mouse brain tissues compared with tissue from young animals. An increased UT\u2010B1 protein abundance was observed in the hippocampus, cerebellum, frontal cortex, and occipital cortex. In contrast, no such changes were observed in the abundance of MCT1 short\u2010chain fatty acid transporters in these aged tissues. These data therefore confirmed that specific increases in UT\u2010B1 protein abundance occur in multiple regions of the aged male mouse brain. Further studies are now needed to determine cell\u2010specific changes and the functional consequence of increased UT\u2010B1 protein in aged brain tissues.<\/jats:p>","DOI":"10.14814\/phy2.70175","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T06:35:11Z","timestamp":1739946911000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Aging increases UT\u2010B urea transporter protein abundance in brains of male mice"],"prefix":"10.14814","volume":"13","author":[{"given":"Farhana","family":"Pinki","sequence":"first","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin  Dublin 4 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren","family":"McKeever","sequence":"additional","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin  Dublin 4 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek A.","family":"Costello","sequence":"additional","affiliation":[{"name":"UCD School of Biomolecular and Biomedical Science University College Dublin  Dublin 4 Ireland"},{"name":"UCD Conway Institute University College Dublin  Dublin 4 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8688-6401","authenticated-orcid":false,"given":"Gavin","family":"Stewart","sequence":"additional","affiliation":[{"name":"UCD School of Biology and Environmental Science University College Dublin  Dublin 4 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10684"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050152"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-024-01076-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(96)00172-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2023.1126615"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1711243115"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2021.591726"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-021-03283-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.05.011"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-011-9594-3"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00286.2004"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2013.07.002"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.10.097"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)31744-7"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2023.1215637"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2023.101563"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.06.009"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2021.09.025"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2021.711396"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-006-0870-z"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01377.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00127.2005"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00359.2001"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00284.2014"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13826"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2012.11.008"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2016.03.001"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/365844a0"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-019-02323-x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci9020073"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.70175","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:17:49Z","timestamp":1741742269000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.70175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.14814\/phy2.70175"],"URL":"https:\/\/doi.org\/10.14814\/phy2.70175","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"type":"print","value":"2051-817X"},{"type":"electronic","value":"2051-817X"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-10-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70175"}}