{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T15:05:01Z","timestamp":1783955101084,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T00:00:00Z","timestamp":1740009600000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>L\u2010Arginine supplementation has beneficial effects on metabolic disorders in rodents. We here investigated the effects of exogenous L\u2010arginine on cardiac pathology and mitochondrial reactive oxygen species (ROS) production and dynamics in DahlS.Z\u2010<jats:italic>Lepr<\/jats:italic><jats:sup><jats:italic>fa<\/jats:italic><\/jats:sup><jats:italic>\/Lepr<\/jats:italic><jats:sup><jats:italic>fa<\/jats:italic><\/jats:sup> (DS\/obese) rats, a model of metabolic syndrome (MetS). DS\/obese rats and their lean homozygous littermate (DahlS.Z\u2010<jats:italic>Lepr<\/jats:italic><jats:sup><jats:italic>+<\/jats:italic><\/jats:sup><jats:italic>\/Lepr<\/jats:italic><jats:sup><jats:italic>+<\/jats:italic><\/jats:sup>, or DS\/lean) controls were provided with drinking water containing 0.50% L\u2010arginine\u2010HCl or 0.85% L\u2010alanine (isonitrogenous control) from 13 to 17\u2009weeks of age. L\u2010Arginine supplementation markedly alleviated hypertension without affecting cardiac injury in MetS rats. It also attenuated the increase in ROS production apparent in cardiac mitochondria isolated from MetS rats as well as suppressed the associated upregulation of Nox4 mRNA and protein in the heart. Furthermore, L\u2010arginine reversed the decrease in the size of cardiac mitochondria as well as changes in the expression of DRP1 and OPA1 proteins apparent in the L\u2010alanine\u2013treated MetS rat heart. Cardiac arginase II gene expression and arginase activity were increased by L\u2010arginine treatment in MetS rats but not CONT rats. L\u2010Arginine supplementation thus ameliorated hypertension and cardiac mitochondrial abnormalities in MetS rats, with the lack of a cardioprotective effect possibly being due to increased arginase activity.<\/jats:p>","DOI":"10.14814\/phy2.70183","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T06:26:12Z","timestamp":1740119172000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["L\u2010arginine ameliorates hypertension and cardiac mitochondrial abnormalities but not cardiac injury in male metabolic syndrome rats"],"prefix":"10.14814","volume":"13","author":[{"given":"Kaito","family":"Tagami","sequence":"first","affiliation":[{"name":"Pathophysiology Sciences, Department of Integrated Health Sciences Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Touko","family":"Okuzawa","sequence":"additional","affiliation":[{"name":"Pathophysiology Sciences, Department of Integrated Health Sciences Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Pathophysiology Sciences, Department of Integrated Health Sciences Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rin","family":"Mishima","sequence":"additional","affiliation":[{"name":"Pathophysiology Sciences, Department of Integrated Health Sciences Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsuki","family":"Obara","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Nagoya University School of Health Sciences  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuko","family":"Kunimatsu","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Nagoya University School of Health Sciences  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayako","family":"Koide","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Nagoya University School of Health Sciences  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamami","family":"Teranishi","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Nagoya University School of Health Sciences  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Itakura","sequence":"additional","affiliation":[{"name":"Division for Medical Research Engineering Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhide","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Pathophysiology Sciences, Department of Integrated Health Sciences Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toyoaki","family":"Murohara","sequence":"additional","affiliation":[{"name":"Department of Cardiology Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0089-6794","authenticated-orcid":false,"given":"Kohzo","family":"Nagata","sequence":"additional","affiliation":[{"name":"Pathophysiology Sciences, Department of Integrated Health Sciences Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41387-020-00132-1"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/hr.2009.153"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1070270303"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.12.002"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00037.2016.\u2010The"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.83"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2010.12.044"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.4172\/2167\u20100943.1000e121"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.214601"},{"key":"e_1_2_12_11_1","first-page":"721","article-title":"Dietary L\u2010arginine supplementation reduces fat mass in Zucker diabetic fatty rats 1","volume":"135","author":"Fu W. 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