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However, existing models often vary in the methods used to induce ischemia, and the resulting AKI tends to be mild\u2010to\u2010moderate. Moreover, follow\u2010up is often performed under volatile anesthesia, which, in contrast to total intravenous anesthesia (TIVA), can induce malignant hyperthermia and cause hemodynamic instability. Here we present a novel surgical model of IR\u2010induced AKI using bilateral renal artery clamping under TIVA. Anesthesia was induced via TIVA with diazepam, ketamine, and morphine. After retroperitoneal exposure, the renal arteries were isolated and clamped with a plastic tube for 90\u2009min, followed by 8\u2009h of reperfusion. The IR group (<jats:italic>n<\/jats:italic>\u2009=\u20096) was compared with a Sham group (<jats:italic>n<\/jats:italic>\u2009=\u20095) that underwent the same procedure without IR. The IR group developed moderate\u2010to\u2010severe AKI as evidenced by reduced glomerular filtration, a 158% increase in plasma creatinine versus 21% in the Sham group, and elevated neutrophil gelatinase\u2010associated lipocalin levels (+280% in IR vs. 0% in Sham), indicating tubular injury. Histopathology confirmed these findings. Thus, this preclinical model successfully induced moderate\u2010to\u2010severe AKI in pigs. The TIVA anesthetic protocol offered several advantages compared to halogenated gas anesthesia.<\/jats:p>","DOI":"10.14814\/phy2.70203","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:54:45Z","timestamp":1738544085000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Temporary bilateral clamping of renal arteries induces ischemia\u2013reperfusion: A new pig model of acute kidney injury using total intravenous anesthesia"],"prefix":"10.14814","volume":"13","author":[{"given":"Axel","family":"Guilpin","sequence":"first","affiliation":[{"name":"MexBrain  Villeurbanne France"},{"name":"Universit\u00e9 de Lyon, UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis, VetAgro Sup  Marcy l'Etoile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathieu","family":"Magnin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis, VetAgro Sup  Marcy l'Etoile France"},{"name":"Universit\u00e9 de Lyon, VetAgro Sup, Unit\u00e9 de Physiologie, Pharmacodynamie et Th\u00e9rapeutique  Marcy l'Etoile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Axel","family":"Aigle","sequence":"additional","affiliation":[{"name":"MexBrain  Villeurbanne France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Yves","family":"Ayoub","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis, VetAgro Sup  Marcy l'Etoile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timoth\u00e9e","family":"Schuhler","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis, VetAgro Sup  Marcy l'Etoile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romain","family":"Lac","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis, VetAgro Sup  Marcy l'Etoile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Marchal","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, VetAgro Sup, Pole de Pathologie V\u00e9t\u00e9rinaire  Marcy l'Etoile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Brichart","sequence":"additional","affiliation":[{"name":"MexBrain  Villeurbanne France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8714-2789","authenticated-orcid":false,"given":"Abdessalem","family":"Hammed","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis, VetAgro Sup  Marcy l'Etoile France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1889-7189","authenticated-orcid":false,"given":"Vanessa","family":"Louzier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis, VetAgro Sup  Marcy l'Etoile France"},{"name":"Universit\u00e9 de Lyon, VetAgro Sup, Unit\u00e9 de Physiologie, Pharmacodynamie et Th\u00e9rapeutique  Marcy l'Etoile France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.34407"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12882\u2010019\u20101678\u20102"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.22317\/jcms.v10i1.1477"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc6863"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2004.04.019"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401236"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c110041"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/s0102\u2010865020160110000008"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.04010907"},{"key":"e_1_2_10_12_1","first-page":"23","article-title":"Regulation of kidney function and metabolism: A question of supply and demand","volume":"118","author":"Blantz R. 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