{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T13:59:49Z","timestamp":1781531989770,"version":"3.54.5"},"reference-count":71,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study evaluated the hepato\u2010nephronal protective properties of <jats:italic>Crataegus azarolus<\/jats:italic> berries decoction extract (CAB\u2010DE) on acetic acid (AA)\u2010induced oxidative stress and metabolic disorders in rats. Animals (60 rats) were randomly divided into six groups (<jats:italic>n<\/jats:italic>\u2009=\u200910), with groups 1 and 2 being controls and groups 3, 4, and 5 given increasing doses of CAB\u2010DE, group 6 were given gallic acid until ulcerative colitis was induced and then intoxicated by an acute intra\u2010rectal infusion of AA. Our results showed that CAB\u2010DE\u2010oral administration had no signs of toxicity or abnormal behavior in rats, with a LD<jats:sub>50<\/jats:sub> higher than 3500\u2009mg\/kg <jats:italic>bw.<\/jats:italic> In addition, CAB\u2010DE protected against AA\u2010induced nephropathy and hepatic damage in rats, as determined by an increase in organ weights and an alteration in the renal and liver parameters and functions. Moreover, extract co\u2010administration reduced AA\u2010induced liver and kidney lipoperoxidation, maintained non\u2010enzymatic contents such as sulfhydryl groups (\u2010SH) and reduced glutathione (GSH), and restored antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). Finally, CAB\u2010DE might have a possible protective effect against AA\u2010oxidative stress and dysfunction in the rat liver and kidney, suggesting that <jats:italic>Crataegus azarolus<\/jats:italic> berries may be beneficial for people suffering from liver issues and nephropathy.<\/jats:p>","DOI":"10.14814\/phy2.70240","type":"journal-article","created":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T00:09:50Z","timestamp":1739146190000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antioxidant activity and protective effect of phyto\u2010active compounds of <i>Crataegus azarolus<\/i> berries decoction extract against acetic acid\u2010induced hepatorenal injuries in male rats"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0449-4098","authenticated-orcid":false,"given":"Houcem","family":"Sammari","sequence":"first","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources, Department of Animal Physiology University of Jendouba, Higher Institute of Biotechnology of Beja  Beja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4619-6892","authenticated-orcid":false,"given":"Anouar","family":"Abidi","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources, Department of Animal Physiology University of Jendouba, Higher Institute of Biotechnology of Beja  Beja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5405-8385","authenticated-orcid":false,"given":"Saber","family":"Jedidi","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources, Department of Animal Physiology University of Jendouba, Higher Institute of Biotechnology of Beja  Beja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nourhen","family":"Dhawefi","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources, Department of Animal Physiology University of Jendouba, Higher Institute of Biotechnology of Beja  Beja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hichem","family":"Sebai","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources, Department of Animal Physiology University of Jendouba, Higher Institute of Biotechnology of Beja  Beja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.21608\/jfds.2021.62433.1016"},{"issue":"1","key":"e_1_2_11_3_1","first-page":"41","article-title":"Hepatoprotective effect of soapworts (Saponaria officinalis), pomegranate peel (Punica granatum L) and cloves (Syzygium aromaticum linn) on mice with CCl4 hepatic intoxication","volume":"1","author":"Abdel\u2010Rahman M. K.","year":"2006","journal-title":"World Journal of Chemistry"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-1729-9"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2016.1205097"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1975.01360130011001"},{"issue":"40","key":"e_1_2_11_8_1","first-page":"9594","article-title":"Comparative analysis of Tunisian wild Crataegus azarolus (yellow azarole) and Crataegus monogyna (red azarole) leaf, fruit, and traditionally derived syrup: Phenolic profiles and antioxidant and antimicrobial activities of the aqueous\u2010acetone extracts","volume":"61","author":"Belkhir M.","year":"2013","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2011.586080"},{"key":"e_1_2_11_10_1","volume-title":"Plantes m\u00e9dicinales d'Alg\u00e9rie","author":"Beloued A.","year":"2005"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.04.012"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00040.2012"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2020.114147"},{"issue":"2","key":"e_1_2_11_14_1","first-page":"345","article-title":"Oxidative processes in red blood cells from normal and diabetic individuals","volume":"37","author":"Bryszewska M.","year":"1995","journal-title":"Biochemistry and Molecular Biology International"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2000.02019.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1986.01400020082010"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X05002606"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf030407y"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3529149"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000354699"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1102942"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)86135-I"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2005.06.002"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000661"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2021.110398"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2012.04.006"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6631415"},{"issue":"5","key":"e_1_2_11_28_1","first-page":"1352","article-title":"Aqueous extract of Solanum anguivi lam. Fruits (African eggplant) inhibit Fe2+ and SNP induced lipid peroxidation in rat's brain\u2010in vitro","volume":"4","author":"Elekofehinti O. O.","year":"2012","journal-title":"Der Pharma Chemica"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/00032710500423385"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21965"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05015-1"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-015-0022-y"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2018.05.058"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1139\/y2012-025"},{"issue":"2","key":"e_1_2_11_36_1","first-page":"83","article-title":"Ascorbic acid, anthocyanins, and phenolics contents and antioxidant activity of ber, azarole, raspberry, and cornelian cherry fruit genotypes growing in Iran","volume":"51","author":"Hashempour A.","year":"2010","journal-title":"Horticulture, Environment and Biotechnology"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.2.675"},{"issue":"9","key":"e_1_2_11_38_1","first-page":"896","article-title":"Antioxidant and hepatoprotective effect of the ethyl acetate extract of Enicostemma axillare (lam). Raynal against CCl 4\u2010induced liver injury in rats","volume":"48","author":"Jaishree V.","year":"2010","journal-title":"Indian Journal of Experimental Biology"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2021.0134"},{"key":"e_1_2_11_40_1","first-page":"130","article-title":"A modified spectrophotometric assay of superoxide dismutase","volume":"2","author":"Kakkar P.","year":"1984","journal-title":"Journal of Biochemistry and Biophysics"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSMBR.905066"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00834.x"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jaoac\/88.5.1269"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nep007"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/00075198-199804000-00006"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/801457"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.21608\/jmh.2019.10441.1052"},{"key":"e_1_2_11_48_1","volume-title":"Encyclop\u00e9die des arbres, plus de 1800 esp\u00e8ces et vari\u00e9t\u00e9s du monde","author":"More D.","year":"2005"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.2298\/ABS170510044M"},{"key":"e_1_2_11_50_1","first-page":"14","article-title":"Test No. 423: Acute oral toxicity\u2010acute toxic class method","volume":"4","author":"OECD","year":"2002","journal-title":"OECD Guidelines for the Testing of Chemicals, Section"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2010.01.002"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.4314\/jasem.v22i4.4"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfoodeng.2004.08.032"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.113.001225"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00702.2012"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.10.030"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3841.2011.02290.x"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1395.041"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2009.10.011"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1006\/jfca.2000.0980"},{"key":"e_1_2_11_61_1","first-page":"1","article-title":"Herbal support for a healthy cardiovascular system","volume":"6","author":"Rose J.","year":"1999","journal-title":"Clinical Nutrition Insight"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1177\/15593258241226890"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.14065"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(68)90092-4"},{"issue":"2","key":"e_1_2_11_65_1","first-page":"144","article-title":"Prevention of acetaminophen induced hepatorenal toxicity in mice with fruits of Terminalia chebula (myrobalan)","volume":"25","author":"Sharma A.","year":"2010","journal-title":"Thai Journal of Toxicology"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2005.07.029"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(57)90063-3"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(02)00338-4"},{"issue":"12","key":"e_1_2_11_69_1","first-page":"1397","article-title":"Quercetin in health and disease","volume":"6","author":"Thennarasu A.","year":"2013","journal-title":"Research Journal of Pharmacy and Technology"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13771"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202306203"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2DT03765C"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.70240","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T09:39:31Z","timestamp":1754905171000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.70240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":71,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.14814\/phy2.70240"],"URL":"https:\/\/doi.org\/10.14814\/phy2.70240","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"value":"2051-817X","type":"print"},{"value":"2051-817X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70240"}}