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The aim of this investigation was to use two dimensional <jats:sup>1<\/jats:sup>H\u2010nuclear magnetic resonance (2D\u2010COSY) metabolomics to identify the chemical changes of kidney fibrogenesis. An in\u00a0vitro transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21)\u2010induced model of kidney fibrogenesis with human kidney\u20102 (HK\u20102) proximal tubular epithelial cells (PTEC) was used. The model was validated by assaying for various pro\u2010fibrotic molecules, using quantitative PCR and Western blotting. 2D\u2010COSY was performed on treated cells. Morphological and functional changes characteristic of tubulointerstitial fibrosis were confirmed in the model; expression of fibronectin, collagen type IV, smooth muscle actin, oxidative stress enzymes increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). NMR metabolomics provided evidence of altered metabolite signatures associated with glycolysis and glutamine metabolism, with decreased myo\u2010inositol and choline, and metabolites of the oxidative phase of the pentose phosphate pathway with increased glucose and glucuronic acid. The altered PTEC cellular metabolism likely supports the rapid fibrogenic energy demands. These results, using 2D\u2010COSY metabolomics, support development of a biomarker panel of fibrosis detectable using clinical magnetic resonance spectroscopy to diagnose and manage CKD.<\/jats:p>","DOI":"10.14814\/phy2.70249","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T05:44:58Z","timestamp":1739771098000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metabolite pathway alterations identified by magnetic resonance metabolomics in a proximal tubular epithelial cell line treated with <scp>TGF<\/scp>\u2010\u03b21"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6735-6886","authenticated-orcid":false,"given":"Tyrone L. 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