{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:12:43Z","timestamp":1753884763826,"version":"3.41.2"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017016","name":"Geriatric Research Education and Clinical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017016","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011649","name":"College of Health Professions, Towson University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011649","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peripheral blood mononuclear cells (PBMCs) represent a heterogeneous mix of cells with paracrine functions that may be altered following prolonged exercise. We determined the effect of ultramarathon running on PBMC paracrine function and PBMC subtype number. Recreational athletes participated in a 50\u2009km ultramarathon. Blood was sampled from <jats:italic>N<\/jats:italic>\u2009=\u20097 at baseline, 10\u2009km, 50\u2009km, and 24\u2009h post\u2010race. PBMCs were isolated and cultured, and conditioned media was used for a HUVEC\u2010based proliferation assay. CD31+, CD3+, and CD31+\/CD3+ PBMCs were quantified at each time point. Proliferation increased from baseline to 50\u2009km (<jats:italic>p<\/jats:italic>\u2009=\u20090.004) and was reduced from 50\u2009km to 24\u2009h post (<jats:italic>p<\/jats:italic>\u2009=\u20090.008). There was an increase in CD31+ PBMCs after 50\u2009km (<jats:italic>p<\/jats:italic>\u2009=\u20090.014), returning to baseline at 24\u2009h post\u2010race (<jats:italic>p<\/jats:italic>\u2009=\u20090.246). CD3+ PBMC and CD31+\/CD3+ PBMC numbers were reduced after 50\u2009km (<jats:italic>p<\/jats:italic>\u2009=\u20090.001 and <jats:italic>p<\/jats:italic>\u2009=\u20090.002, respectively), returning to baseline levels 24\u2009h post\u2010race (<jats:italic>p<\/jats:italic>\u2009=\u20090.190 and <jats:italic>p<\/jats:italic>\u2009=\u20090.315, respectively). PBMC paracrine activity following a 50\u2009km enhances endothelial cell proliferation. Alterations in PBMC subtypes after 50\u2009km suggest a protective role of PBMCs in response to prolonged stresses of ultramarathon running.<\/jats:p>","DOI":"10.14814\/phy2.70255","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T04:33:46Z","timestamp":1740026026000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Peripheral blood mononuclear cell number and paracrine\u00a0function in responses to a 50\u2010km trail race: An\u00a0exploratory study"],"prefix":"10.14814","volume":"13","author":[{"given":"Rian Q.","family":"Landers\u2010Ramos","sequence":"first","affiliation":[{"name":"Department of Kinesiology Towson University  Towson Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Maryland  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Heilman","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Maryland  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William S.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Exercise Science Elon University  Elon North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odessa","family":"Addison","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy and Rehabilitation Science University of Maryland  Baltimore Maryland USA"},{"name":"Department of Veterans Affairs Baltimore Veterans Affairs Medical Center Geriatric Research, Education and Clinical Center  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushant M.","family":"Ranadive","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Maryland  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4627-6489","authenticated-orcid":false,"given":"Steven J.","family":"Prior","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Maryland  College Park Maryland USA"},{"name":"Department of Veterans Affairs Baltimore Veterans Affairs Medical Center Geriatric Research, Education and Clinical Center  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000124104.23702.a0"},{"key":"e_1_2_9_3_1","first-page":"602","article-title":"Circulating progenitor cells decrease immediately after marathon race in advanced\u2010age marathon runners","volume":"15","author":"Adams V.","year":"2008","journal-title":"European Journal of Preventive Cardiology"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20120690"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01344.2009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00376.2002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00450.2013"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.90837.2008"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/157488810793351721"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e318213fefb"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2009.01.003"},{"key":"e_1_2_9_12_1","first-page":"1","article-title":"A novel therapeutic approach using peripheral blood mononuclear cells preconditioned by oxygen\u2010glucose deprivation","volume":"9","author":"Hatakeyama M.","year":"2019","journal-title":"Scientific Reports"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022287"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehs459"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.694778"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000114236.77009.06"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22940"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00541.2011"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.215277"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2010.01.070"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.11.24"},{"volume-title":"The impact of food bio\u2010actives on gut health","year":"2015","author":"Kleiveland C. R.","key":"e_1_2_9_22_1"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2009.10.009"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00601.2010"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0273510"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42978-021-00142-0"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00123.2015"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00342.2016"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085505"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000002955"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00349.2016"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00547-003-0977-3"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00532.2009"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00936.2016"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-018-04065-7"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP091680"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000058702.69484.A0"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2004.02.049"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP092041"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085771"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00592.2017"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.13181\/mji.v25i1.1277"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00477.2019"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1097\/JSA.0000000000000198"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000001029"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hlc.2011.06.003"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.70255","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:19:16Z","timestamp":1741742356000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.70255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.14814\/phy2.70255"],"URL":"https:\/\/doi.org\/10.14814\/phy2.70255","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"type":"print","value":"2051-817X"},{"type":"electronic","value":"2051-817X"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-12-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70255"}}