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The <jats:italic>Ksp\u2010KL<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice have a targeted deletion of <jats:italic>Klotho<\/jats:italic> in the distal part of the nephron. Considering that the distal convoluted tubule is the most important site for Ca<jats:sup>2+<\/jats:sup> regulation in the kidney, <jats:italic>Ksp\u2010KL<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice were challenged with a Ca<jats:sup>2+<\/jats:sup>\u2010deficient diet for determining the Ca<jats:sup>2+<\/jats:sup> handling and pinpointing the <jats:italic>Klotho<\/jats:italic> levels needed for controlling renal Ca<jats:sup>2+<\/jats:sup> handling. The <jats:italic>Ksp\u2010KL<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice displayed normal weight and showed unaltered calcium and phosphate levels in serum and 24\u2010h urine. Expression of calciotropic (<jats:italic>Trpv5<\/jats:italic>, <jats:italic>Trpv6<\/jats:italic>) and phosphotropic (<jats:italic>Slc34a1<\/jats:italic>, <jats:italic>Slc34a2<\/jats:italic>) genes in the kidneys, duodenum, ileum, and colon were not affected by <jats:italic>Klotho<\/jats:italic> deletion. In conclusion, our study reports that mice with 18%\u201393% residual levels of <jats:italic>Klotho<\/jats:italic> in the kidney exhibit normal electrolyte homeostasis when placed on a low Ca<jats:sup>2+<\/jats:sup>\u2010content diet.<\/jats:p>","DOI":"10.14814\/phy2.70297","type":"journal-article","created":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:03:18Z","timestamp":1743552198000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Partial renal deletion of Klotho is not sufficient to impact renal electrolyte handling in distal convoluted tubule specific knock\u2010out mice"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9759-0398","authenticated-orcid":false,"given":"Teodora V.","family":"Grigore","sequence":"first","affiliation":[{"name":"Department of Medical Biosciences Research Institute for Medical Innovation, Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7885-4139","authenticated-orcid":false,"given":"Quinty M.","family":"Leusink","sequence":"additional","affiliation":[{"name":"Department of Medical Biosciences Research Institute for Medical Innovation, Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5071-9000","authenticated-orcid":false,"given":"Malou","family":"Zuidscherwoude","sequence":"additional","affiliation":[{"name":"Department of Medical Biosciences Research Institute for Medical Innovation, Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5016-9435","authenticated-orcid":false,"given":"Caro","family":"Bos","sequence":"additional","affiliation":[{"name":"Department of Medical Biosciences Research Institute for Medical Innovation, Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9911-9201","authenticated-orcid":false,"given":"Hannes","family":"Olauson","sequence":"additional","affiliation":[{"name":"Division of Renal Medicine Department of Clinical Science, Intervention and Technology, Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1816-8544","authenticated-orcid":false,"given":"Joost","family":"Hoenderop","sequence":"additional","affiliation":[{"name":"Department of Medical Biosciences Research Institute for Medical Innovation, Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108665"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13959"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008121273"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","unstructured":"Andrukhova O. 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