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Participants had a mean V\u0307O<jats:sub>2<\/jats:sub>max of 49.8 [SD: 10.9] mL\/kg\/min, hemoglobin mass of 775 [180] g, and red blood cell volume of 2370 [550] mL. After a loading dose, participants ingested <jats:sup>2<\/jats:sup>H<jats:sub>2<\/jats:sub>O daily for 28\u2009days to maintain a stable <jats:sup>2<\/jats:sup>H body water enrichment (~0.5 atom percent excess (APE)), as measured in saliva samples. <jats:sup>2<\/jats:sup>H\u2010enriched alanine APE was measured in RBC protein using gas chromatography pyrolysis isotope ratio mass spectrometry. The increase in APE for Hb protein was nonlinear for the first 2\u2009weeks but stabilized from day 14 to day 28, with mean APE reaching 0.033 [0.005]% on day 28. Hb FSR calculated over this 2\u2010week period was 0.84 [0.15]%\/day, which equated to an Hb absolute synthetic rate of 6.5 [2.2] g\/day and a lifespan of 126 [30] days. Hb FSR was not different between trained (0.83 [0.19] %\/day) and untrained (0.86 [0.24] %\/day, <jats:italic>p<\/jats:italic>\u2009=\u20090.81) individuals or between males (0.80 [0.25] %\/day) and females (0.88 [0.17] %\/day, <jats:italic>p<\/jats:italic>\u2009=\u20090.24). Endurance training and sex did not affect Hb FSR. The <jats:sup>2<\/jats:sup>H<jats:sub>2<\/jats:sub>O method to measure Hb FSR is feasible in humans.<\/jats:p>","DOI":"10.14814\/phy2.70311","type":"journal-article","created":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T11:02:36Z","timestamp":1745060556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hemoglobin synthesis rates in young trained and untrained males and females are not different"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6999-3096","authenticated-orcid":false,"given":"Hilkka","family":"Kontro","sequence":"first","affiliation":[{"name":"Faculty of Kinesiology, Human Performance Lab University of Calgary  Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"McGlory","sequence":"additional","affiliation":[{"name":"School of Kinesiology and Health Studies Queen's University  Kingston Ontario 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