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TFEB, a transcription factor with a myriad of cellular activities, is one of the most abundant genes expressed in syncytiotrophoblasts compared to cytotrophoblasts. TFEB is localized to the nucleus of human BeWo differentiated syncytiotrophoblasts and to the cytoplasm of the undifferentiated cytotrophoblasts. Within both the cytotrophoblasts and syncytiotrophoblasts, TFEB exists in subcellular compartments as both phosphorylated and unphosphorylated forms and translocates between cytoplasm and nucleus upon amino acid starvation\/refeeding. Endogenous TFEB and endogenous phospho\u2010TFEB are both rapidly (<jats:italic>t<\/jats:italic><jats:sub>1\/2<\/jats:sub>\u2009~\u20092\u20133\u2009h) degraded via the ubiquitin proteasome system in cytotrophoblasts and in syncytiotrophoblasts. These results suggest dynamic regulatory processes during trophoblast development\/differentiation.<\/jats:p>","DOI":"10.14814\/phy2.70383","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T04:29:23Z","timestamp":1748233763000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Regulation of <scp>TFEB<\/scp> in human placental Cytotrophoblasts and Syncytiotrophoblasts"],"prefix":"10.14814","volume":"13","author":[{"given":"A.","family":"Mathew","sequence":"first","affiliation":[{"name":"Department of Pediatrics Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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