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The muscle atrophy caused by unloading can be partially attributed to the dysregulation of Ca<jats:sup>2+<\/jats:sup> in the muscle cell, which can activate calpain\u2010mediated proteolysis. The sarco(endo)plasmic reticulum Ca<jats:sup>2+<\/jats:sup>\u2010ATPase (SERCA) is a primary regulator of Ca<jats:sup>2+<\/jats:sup> in muscle, and SERCA dysfunction has been repeatedly demonstrated in various models of muscle unloading. Heat shock protein 70 (HSP70) is a heat\u2010inducible chaperone protein that binds to SERCA and protects against its dysfunction. While previous research has shown HT to upregulate HSP70 in rodent muscle, even in the unloaded state, the effects of HT on SERCA function in rodent skeletal muscle under these conditions remain unknown. Here, we characterized the effects of 4\u2009weeks of HT on soleus muscle size, HSP70 expression, SERCA function, and maximal calpain activity in male C57BL\/6J mice subjected to muscle unloading through tenotomy. Four weeks of HT preserved the cross\u2010sectional area of soleus myofibres following tenotomy, while also upregulating HSP70, maintaining SERCA\u2010mediated Ca<jats:sup>2+<\/jats:sup> uptake, and reducing maximal calpain activity. Therefore, our research offers new insights into the advantages of HT for muscle health and physiology.<\/jats:p>","DOI":"10.14814\/phy2.70385","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T02:40:21Z","timestamp":1747968021000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Heat therapy preserves myofibre size and <scp>SERCA<\/scp>\u2010mediated Ca<sup>2+<\/sup> uptake in the mouse soleus after tenotomy surgery"],"prefix":"10.14814","volume":"13","author":[{"given":"Michael K.","family":"Barfoot","sequence":"first","affiliation":[{"name":"Department of Kinesiology Brock University  St. Catharines Ontario Canada"},{"name":"Centre for Bone and Muscle Health Brock University  St. Catharines Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica L.","family":"Braun","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Brock University  St. Catharines Ontario Canada"},{"name":"Centre for Bone and Muscle Health Brock University  St. Catharines Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip J.","family":"Wallace","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Brock University  St. Catharines Ontario Canada"},{"name":"Centre for Bone and Muscle Health Brock University  St. Catharines Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca M.","family":"Marcella","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Brock University  St. Catharines Ontario Canada"},{"name":"Centre for Bone and Muscle Health Brock University  St. Catharines Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan W.","family":"Baranowski","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Brock University  St. Catharines Ontario Canada"},{"name":"Centre for Bone and Muscle Health Brock University  St. Catharines Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1577-2658","authenticated-orcid":false,"given":"Rebecca E. 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