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Current noninvasive methods like late gadolinium enhancement (LGE) MRI may overestimate infarct size. Manganese\u2010enhanced MRI (MEMRI) emerged as a promising alternative, offering greater specificity in assessing myocardial damage. We evaluated MEMRI alongside LGE and histology in a murine ischemia\u2013reperfusion model using a novel \u201cin\u2010scanner\u201d remote occlusion technique for real\u2010time imaging during acute ischemia. Male C57BL\/6 mice (<jats:italic>n<\/jats:italic>\u2009=\u200916) underwent left anterior descending artery occlusion (<jats:italic>n<\/jats:italic>\u2009=\u20097), sham surgery (<jats:italic>n<\/jats:italic>\u2009=\u20096), or no intervention (<jats:italic>n<\/jats:italic>\u2009=\u20093). MEMRI (0.1\u2009mmol\/kg MnCl<jats:sub>2<\/jats:sub>) during ischemia (0\u201360\u2009min) and LGE (0.1\u2009mmol\/kg Gd\u2010DOTA, 24\u2009h post\u2010surgery) quantified perfusion deficits and infarct size. MEMRI detected acute hypo\u2010perfusion (lateral wall signal reduction: <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01 vs. septal), confined to the occluded territory, while LGE overestimated infarct size (<jats:italic>p<\/jats:italic>\u2009=\u20090.0225 vs. histology). Ischemic mice showed adverse remodeling with reduced ejection fraction (61.37% vs. 71.92%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). MEMRI\u2010derived perfusion deficits correlated with functional decline and histology\u2010confirmed infarcts. Pre\u2010occlusion T<jats:sub>1<\/jats:sub> times did not differ between ischemic and sham groups (<jats:italic>p<\/jats:italic>\u2009=\u20090.85), confirming technique specificity. MEMRI enables early, accurate ischemic injury detection and predicts cardiac dysfunction, outperforming LGE in infarct size determination. Our remote occlusion technique facilitates real\u2010time perfusion assessments, enhancing preclinical myocardial ischemia studies.<\/jats:p>","DOI":"10.14814\/phy2.70442","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T03:55:46Z","timestamp":1751514946000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Manganese\u2010enhanced <scp>MRI<\/scp> during remotely induced myocardial ischemia reperfusion injury in male mice"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5731-4647","authenticated-orcid":false,"given":"Matic","family":"Pusovnik","sequence":"first","affiliation":[{"name":"Biomedical MRI, Department of Imaging and Pathology KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiel","family":"Algoet","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Sciences KU 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