{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T22:21:28Z","timestamp":1785450088715,"version":"3.56.0"},"reference-count":44,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["P01 HL\u201062222"],"award-info":[{"award-number":["P01 HL\u201062222"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Treadmill exercise tests assess aerobic capacity in chronic heart failure (CHF) mice, but subjective exhaustion criteria limit reproducibility. Elevated blood lactate (La) at exhaustion may provide an objective fatigue marker. This study evaluated La at exhaustion and the effects of exercise training (ExT) on resting La in CHF mice. Sham and coronary artery ligation\u2010induced CHF mice were assigned to sedentary (Sed) or ExT groups. Blood La was measured from 0.7\u2009\u03bcL tail vein samples at rest and at exhaustion during exercise tests after 8\u2009weeks of ExT. Blood La at rest was slightly higher in CHF\u2010Sed mice (3.33\u2009\u00b1\u20091.23\u2009mmol\/L) than in Sham\u2010Sed mice (2.96\u2009\u00b1\u20090.42\u2009mmol\/L; <jats:italic>p<\/jats:italic>\u2009=\u20090.86). ExT slightly reduced La in CHF mice (2.55\u2009\u00b1\u20090.63\u2009mmol\/L; <jats:italic>p<\/jats:italic>\u2009=\u20090.34 vs. CHF\u2010Sed), but differences were not significant. At exhaustion, blood La increased significantly across groups. Baseline La in CHF\u2010Sed mice negatively correlated with running distance (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.84, <jats:italic>p<\/jats:italic>\u2009=\u20090.03). Blood La levels at exhaustion offer a cost\u2010effective method to confirm exhaustion criteria and enhance the reliability of exercise tests. La at rest may not reflect CHF severity or exercise adaptation but correlates better with exercise capacity in CHF.<\/jats:p>","DOI":"10.14814\/phy2.70485","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T17:55:25Z","timestamp":1753898125000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Blood lactate as a marker of exercise adaptation and fatigue in male mice with chronic heart failure"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1371-2042","authenticated-orcid":false,"given":"Ahmed M.","family":"Wafi","sequence":"first","affiliation":[{"name":"Basic Medical Science Department, Faculty of Medicine Jazan University  Jazan Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lie","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irving H.","family":"Zucker","sequence":"additional","affiliation":[{"name":"Department of Cellular and Integrative Physiology University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00862.2003"},{"key":"e_1_2_11_3_1","volume-title":"Resource book for the design of animal exercise protocols","author":"American Physiological Society","year":"2006"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.77.2.159"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00991.2004"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2009.8"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1249\/00005768-198502000-00003"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.04.020"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00162.2017"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2009.009165"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2007.04635.x"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00683.2020"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.223925"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2016.00636"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00469.2015"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.38.030176.001421"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00145.2018"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00714.2001"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00231.2002"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00335.2017"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0215584"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.06.002"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1933.106.3.689"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)31071-1"},{"issue":"6","key":"e_1_2_11_25_1","first-page":"H2147","article-title":"Myocardial ischemia and reperfusion: A murine model","volume":"269","author":"Michael L. 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