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Recent studies have reported that the \u03b2\u2010adrenergic receptor inhibited the cell cycle re\u2010entry of ventricular cardiomyocytes. The present study tested the hypothesis that sympathetic recruitment of p38\u03b1\/\u03b2 MAPK signaling suppressed cell cycle re\u2010entry of one or both subpopulations of 1\u2010day\u2010old NNVMs. Norepinephrine (NE; 1\u2009\u03bcM) or Isoproterenol (ISO; 1\u2009\u03bcM) treatment of NNVMs for 24\u2009h significantly attenuated 5\u2010bromo\u20102\u2032\u2010deoxyuridine incorporation, and NE increased p38\u03b1 MAPK phosphorylation. SB203580 (10\u2009\u03bcM) treatment of NE\/ISO\u2010stimulated NNVMs selectively re\u2010established the cell cycle re\u2010entry of the subpopulation distinguished by the absence of de novo nestin expression. Phorbol 12,13\u2010dibutyrate (PDBu; 100\u2009nM) treatment attenuated 5\u2010bromo\u20102\u2032\u2010deoxyuridine incorporation, and SB203580 treatment predominantly initiated re\u2010entry into the cell cycle of the NNVM subpopulation characterized by de novo nestin expression. siRNA\u2010mediated PKC\u2010\u03b1 protein downregulation in NNVMs selectively suppressed PDBu\/SB203580\u2010mediated de novo nestin and subsequent cell cycle re\u2010entry. Thus, sympathetic stimuli acting via the \u03b2<jats:sub>1\/2<\/jats:sub>\u2010adrenergic receptor selectively inhibited the cell cycle re\u2010entry of the nestin<jats:sup>(\u2212)<\/jats:sup>\u2010NNVM subpopulation via recruitment of p38\u03b1\/\u03b2 MAPK.<\/jats:p>","DOI":"10.14814\/phy2.70488","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T19:33:45Z","timestamp":1753904025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Norepinephrine inhibits cell cycle re\u2010entry of neonatal rat ventricular cardiomyocytes characterized by the absence of de novo nestin expression"],"prefix":"10.14814","volume":"13","author":[{"given":"Adrien","family":"Aubry","sequence":"first","affiliation":[{"name":"Montreal Heart Institute Universit\u00e9 de Montr\u00e9al  Montreal Quebec Canada"},{"name":"D\u00e9partement de Pharmacologie et Physiologie Universit\u00e9 de Montr\u00e9al  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