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We previously demonstrated that downregulation of either miR\u2010181a or miR\u2010200c with intracranial injection of an inhibitor is protective against experimental stroke in mice. Here, we generated genetic lines of inducible Ca<jats:sup>2+<\/jats:sup>\u2010calmodulin kinase II\u03b1 (CKII\u03b1) neuronal miR\u2010181a\/b\u20101 and miR\u2010141\/200c cluster deletion to investigate whether the protective effect of their inhibition could be neuron\u2010specific. Jackson Lab strains Mirc14tm1.1Czc\/J and Mirc13tm1Mtm\/Mmjax were each crossed with the tamoxifen\u2010inducible Cre\u2010recombinase strain B6;129S6\u2010Tg, CKII\u03b1\u2010cre\/ERT2. Adult double transgenic male mice were randomized and treated with 3\u2009mg tamoxifen or vehicle via oral gavage for 7\u2009days prior to 1\u2009h middle cerebral artery occlusion (MCAO) or sham surgery. Mice were assessed for gross motor function at 24\u2009h and then sacrificed for quantification of infarct volume. Separate animals were assessed for cell\u2010type specific brain expression of miR\u2010181a and miR\u2010200c via combined fluorescent immunohistochemistry and in\u00a0situ hybridization. Brains from tamoxifen treated mice exhibited selective miR deletion in CKII\u03b1 neurons. Infarct volumes were significantly lower, and neurological scores significantly improved in CKII\u03b1\/miR\u2010200c mice pretreated with tamoxifen versus vehicle alone. In contrast, no difference was observed in infarct volume or neurological score in CKII\u03b1\/miR\u2010181a mice pretreated with tamoxifen versus vehicle.<\/jats:p>","DOI":"10.14814\/phy2.70505","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T13:41:59Z","timestamp":1755178919000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Conditional neuronal deletion of <scp>microRNA<\/scp>\u2010141\/200c cluster, but not <scp>microRNA<\/scp>\u2010181a\/b\u20101 cluster, is protective against experimental stroke in male mice"],"prefix":"10.14814","volume":"13","author":[{"given":"Lijun","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Perioperative and Pain Medicine Stanford University, School of Medicine  Palo Alto California 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