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Within each group, treatment of <jats:italic>n<\/jats:italic>\u2009=\u20093 gilts was initiated at gestational day 34, 45, 55, or 65, and terminated 21\u2009days later to allow for fetal sample collection. The morphology of the fetal thyroid was assessed via lectin staining, showing that follicular structure changes with both increasing gestational age and altered thyroid status. Subsequently, the ontogeny of thyroid and RAS\u2010related genes in the fetal liver and kidney, as well as the effect of fetal hypothyroidism, was assessed via qPCR. In the liver, <jats:italic>SERPINA7<\/jats:italic>, <jats:italic>TTR<\/jats:italic>, <jats:italic>THRA<\/jats:italic>, and <jats:italic>THRB<\/jats:italic> were all ontogenically regulated, with <jats:italic>THRB<\/jats:italic> also ontogenically regulated in the kidney. All studied RAS genes were ontogenically regulated in at least one of the studied tissues. Hypothyroidism caused temporal dysregulations in the expression of hepatic <jats:italic>SERPINA7<\/jats:italic>, <jats:italic>AGT<\/jats:italic>, <jats:italic>ACE<\/jats:italic>, <jats:italic>ACE2<\/jats:italic>, and <jats:italic>AGTR2<\/jats:italic>, as well as renal <jats:italic>THRB<\/jats:italic>, <jats:italic>ACE<\/jats:italic>, and <jats:italic>AGTR1<\/jats:italic>. These results suggest a temporal and tissue\u2010dependent relationship between the thyroid and RAS in the fetal pig.<\/jats:p>","DOI":"10.14814\/phy2.70565","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:06:49Z","timestamp":1757707609000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Gestational age and fetal hypothyroidism Alter the porcine thyroid and local hepatic and renal renin\u2010angiotensin systems"],"prefix":"10.14814","volume":"13","author":[{"given":"Alyssa A.","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Animal Sciences Purdue University  West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8596-3057","authenticated-orcid":false,"given":"J. 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