{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T08:01:10Z","timestamp":1779177670535,"version":"3.51.4"},"reference-count":57,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["24K19491"],"award-info":[{"award-number":["24K19491"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Muscle atrophy and weakness are serious problems associated with sepsis. However, only a few pharmacological interventions are available to date. In this study, myostatin antisense was used to prevent sepsis\u2010induced muscle atrophy and weakness. Sepsis was induced in 8\u2010week\u2010old male C57BL\/6J mice via intraperitoneal injection of 1\u2009mg\/g cecal slurry (CS). Myostatin antisense was injected into the right tibialis anterior muscle. Myostatin mRNA was measured in the tibialis anterior and quadriceps femoris muscles on Day 1. The body weight, grip strength, and cross\u2010sectional area of the tibialis anterior muscle were measured on Day 6. The administration of myostatin antisense decreased myostatin expression on Day 1 in the injected side (0.023\u2009\u00b1\u20090.010 in CS vs. 0.008\u2009\u00b1\u20090.002 in CS\u2009+\u2009antisense) as well as in the noninjected muscles. It also decreased the myostatin protein level (2.0\u2009\u00b1\u20090.3 in CS vs. 1.2\u2009\u00b1\u20090.5 in CS\u2009+\u2009antisense, <jats:italic>p<\/jats:italic>\u2009=\u20090.04). Body weight reduction (94.9%\u2009\u00b1\u20092.0% vs. 98.2%\u2009\u00b1\u20091.8%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) and grip strength reduction (77.0%\u2009\u00b1\u200912.3%vs. 89.8%\u2009\u00b1\u20098.3%, <jats:italic>p<\/jats:italic>\u2009=\u20090.04) were suppressed by the injection. The cross\u2010sectional area of the right tibialis anterior muscle increased after the treatment (1116\u2009\u00b1\u2009530\u2009\u03bcm<jats:sup>2<\/jats:sup> vs. 1435\u2009\u00b1\u2009648\u2009\u03bcm<jats:sup>2<\/jats:sup>, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Myostatin antisense suppressed the elevation of myostatin mRNA expression in whole muscles of mice with sepsis and prevented sepsis\u2010induced muscle atrophy and weakness.<\/jats:p>","DOI":"10.14814\/phy2.70566","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:06:46Z","timestamp":1757707606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Myostatin antisense administration prevents sepsis\u2010induced muscle atrophy and weakness in male mice"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2394-2688","authenticated-orcid":false,"given":"Nobuto","family":"Nakanishi","sequence":"first","affiliation":[{"name":"Division of Disaster and Emergency Medicine, Department of Surgery Related Kobe University Graduate School of Medicine  Kobe Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Maeta","sequence":"additional","affiliation":[{"name":"Quality Assurance Section, Pharmaceutical Quality Assurance Dept. KNC Laboratories Co., Ltd.  Izumo Shimane Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Ono","sequence":"additional","affiliation":[{"name":"Division of Disaster and Emergency Medicine, Department of Surgery Related Kobe University Graduate School of Medicine  Kobe Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takumi","family":"Hirabayashi","sequence":"additional","affiliation":[{"name":"Division of Rehabilitation Medicine Kobe University Hospital  Kobe Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Tatebayashi","sequence":"additional","affiliation":[{"name":"Division of Rehabilitation Sapporo Medical University Hospital  Sapporo Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kensuke","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Division of Disaster and Emergency Medicine, Department of Surgery Related Kobe University Graduate School of Medicine  Kobe Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeaki","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Emergency and Critical Care Medicine Wakayama Medical University  Wakayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Graduate School of Science, Technology and Innovation Kobe University  Kobe Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joji","family":"Kotani","sequence":"additional","affiliation":[{"name":"Division of Disaster and Emergency Medicine, Department of Surgery Related Kobe University Graduate School of Medicine  Kobe Hyogo 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