{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:16:40Z","timestamp":1792185400200,"version":"4.4.0"},"reference-count":46,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01\u2010DK104708"],"award-info":[{"award-number":["R01\u2010DK104708"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The effects of TRPC6 activation for various periods of time on three classes of functional outputs were examined in cultured podocytes: albumin permeation across a confluent layer; changes in nephrin dynamics; and cell death. Albumin permeation in transwell assays was significantly increased within 1\u2009h in response to the activation of formyl peptide receptors (FPR), but the TRPC6 inhibitor SAR\u20107334 had no effect on this response, and 1\u2009h or 24\u2009h exposures to the TRPC6 activator PPZ2 did not increase albumin permeation. Direct TRPC6 activation for 24\u2009h evoked an increase in shedding of nephrin ectodomains into the surrounding media, accompanied by an increase in matrix metalloprotease\u20107 (MMP\u20107). These effects were blocked by the calcineurin inhibitor cyclosporin A (CsA), as well as by Batimastat, a broad\u2010spectrum inhibitor of metalloproteinases including MMP\u20107. TRPC6 activation for 24\u2009h also evoked an increase in occludin abundance but had no effect on the abundance of podocin. Finally, TRPC6 activation for 72\u2009h, but not for 24\u2009h, evoked an increase in apoptotic cell death based on increases in cleaved caspase\u20103. This effect was blocked by both SAR\u20107334 and CsA. TRPC6 activation did not induce pyroptosis based on the measurement of cleaved gasdermin D.<\/jats:p>","DOI":"10.14814\/phy2.70614","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T12:14:37Z","timestamp":1761826477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>TRPC6<\/scp>\n                    effects on albumin permeation, nephrin shedding, and apoptosis in podocytes: Role of calcineurin and metalloproteases"],"prefix":"10.14814","volume":"13","author":[{"given":"Eun Young","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biology and Biochemistry University of Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4265-104X","authenticated-orcid":false,"given":"Stuart E.","family":"Dryer","sequence":"additional","affiliation":[{"name":"Department of Biology and Biochemistry University of Houston  Houston Texas USA"},{"name":"Department of Biomedical Sciences, Tilman J. 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