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This study investigates how mitochondrial dysfunction could contribute to CIP by examining metabolic changes in dorsal root ganglia (DRG) culture from newborn Wistar rats exposed to different concentrations of lipopolysaccharide (5, 10, 50, and 100\u2009\u03bcg\/mL for 24 or 48\u2009h). Cell viability was assessed using the MTS assay, gene expression related to inflammation and mitochondrial function was analyzed by Real\u2010Time PCR. IL\u20106 levels of supernatants were measured by ELISA and energetic metabolism was evaluated with the Seahorse MitoStress kit. Exposure to LPS at varying concentrations mainly did not affect cell viability, except at 10\u2009\u03bcg\/mL for 48\u2009h, where a 7.5% increase was noted. Gene expression analysis showed trends in SOD2 and Fis1, with significant increases in IL\u20106 and LIF transcripts at 5 and 10\u2009\u03bcg\/mL. IL\u20106 release was significant at 50 and 100\u2009\u03bcg\/mL of LPS. Mitochondrial respiration and glycolytic metabolism exhibited significant changes in oxygen consumption rate and extracellular acidification, particularly at higher LPS concentrations. The findings suggest that LPS induces an inflammatory environment which led to metabolic disturbances in DRG cells, with adaptive responses at 10 and 50\u2009\u03bcg\/mL of LPS.<\/jats:p>","DOI":"10.14814\/phy2.70633","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T05:59:19Z","timestamp":1762149559000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic changes in dorsal root ganglia of newborn Wistar rats following\n                    <scp>LPS<\/scp>\n                    exposure to understand mechanisms of critical illness polyneuropathy"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3025-7038","authenticated-orcid":false,"given":"Amandine","family":"Szczesnowski","sequence":"first","affiliation":[{"name":"ORPHY Univ Brest  Brest France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christelle","family":"Le 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