{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T03:18:57Z","timestamp":1777087137460,"version":"3.51.4"},"reference-count":66,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Ba 6205\/1 1"],"award-info":[{"award-number":["Ba 6205\/1 1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The genetic predisposition to high glucose\u2010induced peritoneal membrane (PM) injury during peritoneal dialysis (PD) and its mechanisms are of substantial clinical interest. We compared PD\u2010induced peritoneal injury between two closely related mouse substrains, C57BL\/6J and C57BL\/6N, which differ in the function of the mitochondrial enzyme nicotinamide nucleotide transhydrogenase (NNT).\n                    <jats:italic>Nnt<\/jats:italic>\n                    (+\/+) C57BL\/6N mice exhibited significantly greater susceptibility, as indicated by mesothelial cell loss, fibrosis, neoangiogenesis, inflammation, M1 macrophage infiltration, and reduced ultrafiltration. To further investigate NNT's role, we silenced NNT in\u00a0vitro. Knockdown prevented mitochondrial ROS accumulation, reduced pro\u2010inflammatory mediator release in mesothelial cells, inhibited M1 polarization in macrophages, and impaired fibroblast proliferation under high glucose. We also observed a reverse NNT reaction in fibroblasts, contributing to glucose\u2010induced ROS. Our findings indicate reduced genetic susceptibility of\n                    <jats:italic>Nnt<\/jats:italic>\n                    (\u2212\/\u2212) C57BL\/6J mice to PD\u2010induced PM damage and identify NNT as a potential therapeutic target for PD\u2010associated peritoneal injury.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70642","type":"journal-article","created":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T04:53:48Z","timestamp":1763182428000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Nicotinamide nucleotide transhydrogenase deficiency and genetic susceptibility to high glucose\u2010mediated peritoneal injury in mice"],"prefix":"10.14814","volume":"13","author":[{"given":"Margarete C.","family":"Ohse","sequence":"first","affiliation":[{"name":"Department of Nephrology and Hypertension Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Rong","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Phenos GmbH  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S.","family":"Balzer","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension Hannover Medical School  Hannover Germany"},{"name":"Medical Department, Division of Nephrology and Internal Intensive Care Medicine Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Nikolin","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Transplant Engineering Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibylle","family":"von Vietinghoff","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension Hannover Medical School  Hannover Germany"},{"name":"Nephrology Section, Medical Clinic 1 University Hospital Bonn and Bonn University  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"Haller","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension Hannover Medical School  Hannover Germany"},{"name":"Mount Desert Island Biological Laboratory  Salsbury Cove Maine USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai M.","family":"Schmidt\u2010Ott","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulia","family":"Kiyan","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3772-4136","authenticated-orcid":false,"given":"Nelli","family":"Shushakova","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension Hannover Medical School  Hannover Germany"},{"name":"Phenos GmbH  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2005.08.011"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010007\u20100814\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfy282"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10111573"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0201855"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2018\u201000014"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2013.100"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020967"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15\u20100982"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2021.0111"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.2337\/db06\u20100358"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.2147\/JPR.S164493"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/089686080002002S04"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202048733"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2018.12.030"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.04.035"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.2c00713"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/642793"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/089686080202200103"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.321"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101519\u20102"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2014.00123"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP280572"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2021010080"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.885802"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.12.002"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2012.132"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.12.023"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/5835072"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfs289"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23094831"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE\u201016\u20100638"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.58.141"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.20\u20100158"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2019.11.001"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.07.008"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101674"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523\u20101755.2003.00290.x"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.krcp.2016.07.003"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00274.2019"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12865\u2010018\u20100261\u20100"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2009.02.012"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11112184"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10030447"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00607.2020"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.730473"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10050768"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2006.03.010"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2017.04.004"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1177\/08968608211004683"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aav5341"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102013\u201014\u20107\u2010r82"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.84921"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.11.022"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1159\/000514550"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010005\u20101680\u2010z"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2016.01.025"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.01.011"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22116101"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/134708"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2016.09.013"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2011.12.004"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13615"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000002717"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.321"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13464"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.70642","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T09:29:14Z","timestamp":1764149354000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.70642"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":66,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.14814\/phy2.70642"],"URL":"https:\/\/doi.org\/10.14814\/phy2.70642","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"value":"2051-817X","type":"print"},{"value":"2051-817X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"2025-09-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70642"}}