{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:21:02Z","timestamp":1791807662864,"version":"4.3.4"},"reference-count":70,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01 AG071746"],"award-info":[{"award-number":["P01 AG071746"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Menopause leads to loss of cardiovascular and renal protection, and while hormone therapy offers benefits, its efficacy may depend on health status at menopause onset. We hypothesized that preexisting hypertension blunts the renal, cardiac, and vascular effects of Estradiol (E2). Female Long\u2010Evans rats were ovariectomized (OVX) at 46\u2009weeks to model menopause and received either E2 or vehicle, and some were infused with angiotensin II (ANG; 700\u2009ng\/kg\/min) 4\u2009weeks before OVX. Blood pressure (BP) was measured by tail cuff, renal function by urine collection, collagen deposition by histology, and mRNA expression in aorta and kidney by droplet digital PCR. ANG increased BP and proteinuria (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.02), water intake (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), urinary output, heart weight, and aortic NOX4 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), confirming hypertension and oxidative stress. E2 reduced body weight (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.02), increased bone mineral content (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.01), and prevented uterine atrophy (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), confirming E2 treatment. While E2 attenuated cardiac hypertrophy (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.004), it exacerbated proteinuria, decreased GFR (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), and failed to reduce aortic NOX4. ANG did not affect tissue estrogen receptor expression, while E2 showed tissue\u2010specific regulation of GPER and ER\u03b1. In this hypertensive OVX model, E2 failed to protect renal and vascular damage, emphasizing the importance of cardiovascular health at menopause when considering hormone therapy.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70646","type":"journal-article","created":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T05:49:37Z","timestamp":1762580977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Estradiol is not protective against angiotensin II\u2010induced hypertension in middle\u2010aged ovariectomized rats"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4321-4725","authenticated-orcid":false,"given":"A. P. O.","family":"Leite","sequence":"first","affiliation":[{"name":"Department of Pharmacology Tulane School of Medicine  New Orleans Louisiana USA"},{"name":"Tulane Brain Institute  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Pires dos Santos","sequence":"additional","affiliation":[{"name":"Tulane Brain Institute  New Orleans Louisiana USA"},{"name":"Department of Psychology Tulane School of Science &amp; Engineering  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Zha","sequence":"additional","affiliation":[{"name":"Tulane Brain Institute  New Orleans Louisiana USA"},{"name":"Department of Psychology Tulane School of Science &amp; Engineering  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. A.","family":"Blessinger","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Tulane School of Medicine  New Orleans Louisiana USA"},{"name":"Tulane Brain Institute  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Petillo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Tulane School of Medicine  New Orleans Louisiana USA"},{"name":"Tulane Brain Institute  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Jasti","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Tulane School of Medicine  New Orleans Louisiana USA"},{"name":"Tulane Brain Institute  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. C.","family":"Cheeran","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Tulane School of Medicine  New Orleans Louisiana USA"},{"name":"Tulane Brain Institute  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Menon","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Tulane School of Medicine  New Orleans Louisiana USA"},{"name":"Tulane Brain Institute  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. B.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Tulane School of Medicine  New Orleans Louisiana USA"},{"name":"Tulane Brain Institute  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Daniel","sequence":"additional","affiliation":[{"name":"Tulane Brain Institute  New Orleans Louisiana USA"},{"name":"Department of Psychology Tulane School of Science &amp; Engineering  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9837-4582","authenticated-orcid":false,"given":"S. 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