{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T08:33:21Z","timestamp":1782462801873,"version":"3.54.5"},"reference-count":17,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pulmonary congestion is a common complication in critically ill patients, but its quantification remains challenging. The wet\u2010to\u2010dry (W\/D) lung weight ratio, widely used in experimental models, is a terminal and semi\u2010quantitative method. Lung ultrasonography (LU) may offer a non\u2010invasive and reproducible alternative for assessing pulmonary edema. To evaluate the accuracy and reproducibility of LU compared with the W\/D ratio in a rat model of oleic acid\u2013induced lung injury. Thirty Wistar\u2010Kyoto rats were randomized into injury (oleic acid,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200915) and control (saline,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200915) groups. Echocardiography and LU were performed at baseline and 1\u2009h after infusion. B\u2010line scoring was independently analyzed by two echocardiographers, with reproducibility assessed by Bland\u2013Altman plots. Evans blue dye evaluated vascular permeability. Oleic acid\u2013treated rats showed significantly higher LU scores, B\u2010line counts, W\/D ratios, and Evans blue levels than controls. LU demonstrated good diagnostic performance in detecting lung edema in this experimental model, with excellent inter\u2010 and intraobserver agreement confirming strong reproducibility. LU proved accurate, reproducible, and non\u2010terminal for detecting pulmonary congestion in rats, showing good agreement with traditional gravimetric measures and supporting its use for longitudinal assessment of pulmonary edema in experimental research.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70701","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T06:39:34Z","timestamp":1765435174000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pulmonary ultrasound provides a more accurate assessment of pulmonary congestion than wet\/dry lung weight in rats"],"prefix":"10.14814","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3357-7757","authenticated-orcid":false,"given":"Andr\u00e9 Tim\u00f3teo","family":"Sapalo","sequence":"first","affiliation":[{"name":"Division of Emergency Medicine, Department of Internal Medicine, Vascular Biology Laboratory, School of Medicine S\u00e3o Paulo University  Ribeir\u00e3o Preto SP Brazil"},{"name":"HCRP\u2010USP Echocardiography Laboratory, Department of Clinical Medicine, FMRP\u2010USP, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.127.5.1690"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13089\u2010017\u20100068\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aet076"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0000000000000042"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201205\u20100939OC"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054\u2010015\u20100756\u20105"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2010.09.003"},{"key":"e_1_2_11_9_1","first-page":"4","article-title":"Assessment of current methods quantitating extravascular lung water and pulmonary aeration in inhomogeneous lung injury: an experimental study","volume":"3","author":"Kuz'kov V. 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