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Methionine restriction recapitulates many caloric restriction metabolic effects and increases liver expression of activating transcription factor 4 (ATF4) and downstream targets with anti\u2010contractile properties such as cystathionine\u2010\u03b2\u2010synthase (CBS) and cystathionine\u2010\u03b3\u2010lyase (CSE). We hypothesized that dietary methionine restriction would prevent high\u2010fat diet\u2010induced PVAT dysfunction by increasing ATF4 expression. Male Wistar rats were fed control (C, 10% calories from fat) or high\u2010fat diets (H, 60% calories from fat) (HFD) that were methionine replete (R, 0.86% methionine) or deplete (D, 0.12% methionine) for 12\u2009weeks. Methionine restriction prevented body mass increases, independent of fat content, and had no effect on blood pressure or arterial contraction to norepinephrine (10\u2009\u03bcM) or relaxation to acetylcholine (10\u2009\u03bcM). In this feeding paradigm, HFD did not induce PVAT dysfunction. CR diet increased ATF4 transcript levels in multiple PVAT depots but did not consistently affect expression of downstream targets. Protein expression of ATF4 and its targets varied largely for all groups. In conclusion, 12\u2009week methionine restriction profoundly affected body mass but not blood pressure or arterial reactivity in the presence or absence of PVAT.<\/jats:p>","DOI":"10.14814\/phy2.70702","type":"journal-article","created":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T07:39:37Z","timestamp":1766216377000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary methionine depletion and hydrogen sulfide\u2010producing genes in perivascular adipose tissue of male Wistar rats"],"prefix":"10.14814","volume":"13","author":[{"given":"Adam","family":"Corken","sequence":"first","affiliation":[{"name":"Department of Pediatrics University of Arkansas for Medical Sciences  Little Rock Arkansas USA"},{"name":"Arkansas Children's Research Institute  Little Rock Arkansas 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