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Muscle disuse reduces mitochondrial content; however, its effect on mitochondrial subcellular location is unclear. Omega\u20103 fatty acid (n\u20103) attenuates declines in muscle mass and mitochondrial function during disuse; however, whether n\u20103 supplementation prevents the decline in mitochondrial content has not been examined. We investigated the effects of 2\u2009weeks of leg immobilization followed by 2\u2009weeks of remobilization on skeletal muscle mitochondrial content and subcellular localization with and without n\u20103 supplementation. Twenty healthy females supplemented with n\u20103 (2.97\u2009g EPA and 2.03\u2009g DHA) or control (isoenergetic sunflower oil) during 2\u2009weeks of unilateral leg immobilization and 2\u2009weeks of remobilization.\n                    <jats:italic>Vastus lateralis<\/jats:italic>\n                    biopsies were taken for electron microscopic analysis of mitochondrial content. Subsarcolemmal (SS) mitochondrial content decreased during immobilization (control: \u22129%, n\u20103: \u221266%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.009) and remained lower following recovery (control: \u221241%, n\u20103: \u221242%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.005). This effect was driven by the n\u20103 group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.02). Intermyofibrillar (IMF) mitochondrial content did not decline during immobilization, but was lower than baseline following recovery in the central (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.01) IMF. The effects of leg immobilization on mitochondrial content differ by location, are not reversed with short\u2010term recovery, and are influenced by n\u20103 supplementation.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70736","type":"journal-article","created":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T06:26:46Z","timestamp":1769236006000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Omega\u20103 fatty acid supplementation does not attenuate declines in skeletal muscle mitochondrial area in young, healthy females during immobilization"],"prefix":"10.14814","volume":"14","author":[{"given":"Megan M.","family":"Lo","sequence":"first","affiliation":[{"name":"Department of Kinesiology and Health Sciences University of Waterloo  Waterloo Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merryl N.","family":"Black","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Health Sciences University of Waterloo  Waterloo Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"McGlory","sequence":"additional","affiliation":[{"name":"Department of Kinesiology McMaster University  Hamilton Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravninder","family":"Bahniwal","sequence":"additional","affiliation":[{"name":"Department of Kinesiology McMaster University  Hamilton Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Kinesiology McMaster University  Hamilton Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joe","family":"Quadrilatero","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Health Sciences University of Waterloo  Waterloo Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1956-4098","authenticated-orcid":false,"given":"Stuart M.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Kinesiology McMaster University  Hamilton Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9210-8734","authenticated-orcid":false,"given":"Michaela C.","family":"Devries","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Health Sciences University of Waterloo  Waterloo Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1591672"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","unstructured":"Canadian Institutes of Health Research Natural Sciences and Engineering Research Council of Canada Social Sciences and Humanitites Research Council of Canada. 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