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We investigated how repeated heat exposure affects aquaporin\u20104 (AQP4) in skeletal muscle using both in\u00a0vivo and in\u00a0vitro models. Male C57BL\/6NJ mice were exposed to daily passive heating (45\u00b0C for 30\u2009min\/day, 14\u2009days). Repeated heat exposure reduced body\u2010weight gain and markedly attenuated\n                    <jats:italic>Hspa1a<\/jats:italic>\n                    induction following an acute heat challenge, confirming systemic heat acclimation. Immunofluorescence analysis revealed an approximately 1.3\u2010fold increase in AQP4 protein in fast\u2010twitch muscles without changes in its subcellular localization. In\u00a0vitro, C2C12 myocytes were subjected to repeated heat bouts (42\u00b0C, 3\u2009h\/day for 1\u20135\u2009days). Heat\u2010acclimated cells exhibited blunted\n                    <jats:italic>Hspa1a<\/jats:italic>\n                    induction in response to acute heat stress and progressively increased AQP4 protein levels (approximately 1.6\u2010fold), indicating that heat stress directly promotes AQP4 upregulation in muscle cells. Together, these findings identify AQP4 as a heat\u2010responsive component of skeletal muscle and suggest that AQP4 may contribute to peripheral osmotic regulation during heat acclimation.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70926","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T04:41:12Z","timestamp":1779252072000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Repeated heat exposure upregulates skeletal muscle aquaporin\u20104 expression in male mice"],"prefix":"10.14814","volume":"14","author":[{"given":"Yuka","family":"Kudo","sequence":"first","affiliation":[{"name":"Department of Life Sciences, Graduate School of Life Sciences Toyo University  Asaka Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomi","family":"Yazawa","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Graduate School of Life 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