{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T07:05:40Z","timestamp":1779951940213,"version":"3.53.1"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Liver fibrosis is a growing global health problem with no effective treatments. PDGFR\u03b1\u2010positive fibroblasts are the main drivers of liver fibrosis, but their cellular heterogeneity is not fully understood. In this study, we performed single\u2010cell RNA sequencing (scRNA\u2010seq) on PDGFR\u03b1\u2010positive fibroblasts isolated from healthy mouse livers. We also induced liver fibrosis via bile duct ligation (BDL) and examined their interactions with immune cells. scRNA\u2010seq revealed that PDGFR\u03b1\u2010positive fibroblasts mainly consist of hepatic stellate cells and portal fibroblasts. CellChat analysis indicated that a COX2\u2010high (\n                    <jats:italic>Ptgs2<\/jats:italic>\n                    \u2010high) subpopulation may preferentially interact with immune cells. This COX2\u2010positive PDGFR\u03b1\u2010positive fibroblast subset, also reported in other organs, localizes around bile ducts in the liver. Furthermore, when FACS\u2010sorted PDGFR\u03b1\u2010positive fibroblasts were treated with IL\u20101\u03b2 in\u00a0vitro, the proportion of COX2\u2010positive cells increased. These findings demonstrate that PDGFR\u03b1\u2010positive fibroblasts exhibit diversity in their responses to immune signals, with the COX2\u2010positive subset potentially playing a key role in early fibrotic responses. Clarifying this heterogeneity may improve understanding of early liver fibrosis and support development of new therapeutic strategies.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70938","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:38:13Z","timestamp":1779691093000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>COX2<\/scp>\n                    <sup>+<\/sup>\n                    \/\n                    <scp>PDGFR\u03b1<\/scp>\n                    <sup>+<\/sup>\n                    fibroblasts selectively localize near bile ducts and interact with immune cells in early liver fibrosis in mice"],"prefix":"10.14814","volume":"14","author":[{"given":"Momo","family":"Goto","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Pharmacology, Department of Veterinary Medical Sciences, Graduate School of Agriculture and Life Sciences The University of 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