{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T07:08:35Z","timestamp":1783840115506,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102020\u201006809"],"award-info":[{"award-number":["RGPIN\u20102020\u201006809"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002914","name":"Concordia University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002914","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Transwell co\u2010culture systems are models for examining cellular crosstalk, but the impact of porous membrane material on myogenic outcomes remains uncharacterized. We investigated the influence of transwell membrane material on myoblast differentiation in co\u2010culture with human primary adipocytes and in varying concentrations of insulin\u2010transferrin\u2010selenium (ITS). Primary human myoblasts were differentiated on 0.4\u2009\u03bcm polycarbonate or polyester membranes, either alone (Myo\u2010Only) or with adipocytes (Myo\u2009+\u2009Adipo) in two different ITS concentrations ([ITS]; 0.5\u00d7 or 1\u00d7). Myogenesis was evaluated by immunofluorescence, using fusion index (%) and myotube thickness (\u03bcm), and adipokine levels were measured in each co\u2010culture compartment. Polycarbonate membranes supported a greater fusion index and myotube thickness than polyester at both 1\u00d7 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) and 0.5\u00d7 ITS (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.002). Across [ITS] (1\u00d7\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.035; 0.5\u00d7\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.006), the fusion index was higher in Myo\u2009+\u2009Adipo co\u2010cultures than in Myo\u2010Only conditions. Leptin diffusion was reduced across polyester compared to polycarbonate membranes (0.5\u00d7\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.036; 1\u00d7\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.091), whereas adiponectin diffusion was similar between materials. These findings underscore that membrane material and medium composition are critical variables in transwell adipocyte\u2010myoblast co\u2010culture systems. This study highlights the importance in the selection and reporting of these parameters to improve reproducibility across future studies.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70939","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T05:49:03Z","timestamp":1780984143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transwell membrane material affects myogenic differentiation in human primary myoblast\u2010adipocyte co\u2010culture"],"prefix":"10.14814","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0169-9533","authenticated-orcid":false,"given":"Claire","family":"Plissonneau","sequence":"first","affiliation":[{"name":"Department of Health, Kinesiology, and Applied Physiology Concordia University  Montr\u00e9al Quebec Canada"},{"name":"Metabolism, Obesity, and Nutrition Lab, School of Health Concordia University  Montreal Quebec Canada"},{"name":"Centre de Recherche\u2010Axe Maladies Chroniques, CIUSSS\u2010NIM H\u00f4pital du Sacr\u00e9\u2010Coeur de Montr\u00e9al  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6740-8674","authenticated-orcid":false,"given":"Bjorn T.","family":"Tam","sequence":"additional","affiliation":[{"name":"Academy of Wellness and Human Development, Faculty of Arts and Social Sciences Hong Kong Baptist University  Hong Kong SAR China"},{"name":"Dr. Stephen Hui Research Centre for Physical Recreation and Wellness, Faculty of Arts and Social Sciences Hong Kong Baptist University  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1037-8088","authenticated-orcid":false,"given":"Pierre Y.","family":"Garneau","sequence":"additional","affiliation":[{"name":"D\u00e9partement Du Chirurgie H\u00f4pital Du Sacr\u00e9\u2010Coeur de Montr\u00e9al  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9366-6626","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Morais","sequence":"additional","affiliation":[{"name":"Department of Health, Kinesiology, and Applied Physiology Concordia University  Montr\u00e9al Quebec Canada"},{"name":"Division of Geriatric Medicine, Department of Medicine McGill University, McGill University Health Centre (MUHC)\u2010Montreal General Hospital  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4264-6980","authenticated-orcid":false,"given":"Alisa","family":"Piekny","sequence":"additional","affiliation":[{"name":"Department of Biology Concordia University  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5814-6086","authenticated-orcid":false,"given":"Sylvia","family":"Santosa","sequence":"additional","affiliation":[{"name":"Department of Health, Kinesiology, and Applied Physiology Concordia University  Montr\u00e9al Quebec Canada"},{"name":"Metabolism, Obesity, and Nutrition Lab, School of Health Concordia University  Montreal Quebec Canada"},{"name":"Centre de Recherche\u2010Axe Maladies Chroniques, CIUSSS\u2010NIM H\u00f4pital du Sacr\u00e9\u2010Coeur de Montr\u00e9al  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010physiol\u2010042324\u2010100259"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201000335\u20101"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097\u20104652(200102)186:2<183::AID\u2010JCP1020>3.0.CO;2\u2010Q"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12195\u2010020\u201000624\u20101"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743\u20107075\u20108\u201091"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10281"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7LC01248A"},{"key":"e_1_2_10_9_1","unstructured":"Corning. 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