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Aging and cardiovascular disease (CVD) can compromise eGC integrity, potentially preceding conventional indicators of vascular dysfunction such as reduced endothelium\u2010dependent vasodilation and increased arterial stiffness. SDC\u20101 and HS are key glycosaminoglycans and validated plasma biomarkers reflecting glycocalyx integrity. During menopause the decline in estrogen, a vascular\u2010protective hormone, has been linked to increased glycocalyx degradation and decreased nitric oxide (NO) synthesis contributing to endothelial dysfunction. This study examined glycocalyx degradation in premenopausal females versus postmenopausal females (early and late stages). Resting plasma samples from premenopausal (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200911), early\u2010postmenopausal (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912) and late\u2010postmenopausal women (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200913) were analyzed via ELISA for SDC\u20101 and HS. Late postmenopausal females had significantly higher mean plasma HS concentrations compared to early postmenopausal and premenopausal groups (4049.9 \u00b1\u20091595.4 vs. 3919.0 \u00b1\u2009999.2, 2986.4 \u00b1\u20091493.7\u2009ng\/mL,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.003). Conversely, SDC\u20101 levels were higher in premenopausal women (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.036). No correlations were observed between these biomarkers and flow\u2010mediated dilation (all,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Overall, results suggest eGC integrity varies across reproductive stages, emphasizing the need for further research into mechanisms and interventions to preserve vascular health in aging females.\n                  <\/jats:p>","DOI":"10.14814\/phy2.70964","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:15:10Z","timestamp":1782281710000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring Glycocalyx shedding markers: A comparative analysis of plasma markers among premenopausal and postmenopausal women"],"prefix":"10.14814","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9261-5501","authenticated-orcid":false,"given":"Mounica","family":"Ayyalasomayajula","sequence":"first","affiliation":[{"name":"Department of Kinesiology The Pennsylvania State University  University Park Pennsylvania 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jigar","family":"Gosalia","sequence":"additional","affiliation":[{"name":"Department of Kinesiology The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3183-0707","authenticated-orcid":false,"given":"Jocelyn M.","family":"Spicuzza","sequence":"additional","affiliation":[{"name":"Integrative and Biomedical Physiology, Huck Institutes of the Life Sciences The Pennsylvania State University  University Park Pennsylvania USA"},{"name":"Department of Psychiatry University of Pittsburgh School or Medicine  Pittsburgh Pennsylvania 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