{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:01:33Z","timestamp":1790020893497,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL153101"],"award-info":[{"award-number":["HL153101"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL134850"],"award-info":[{"award-number":["HL134850"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK133121"],"award-info":[{"award-number":["DK133121"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL007852"],"award-info":[{"award-number":["HL007852"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32HL134643"],"award-info":[{"award-number":["T32HL134643"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100021528","name":"Advancing a Healthier Wisconsin Endowment","doi-asserted-by":"publisher","award":["9520639"],"award-info":[{"award-number":["9520639"]}],"id":[{"id":"10.13039\/100021528","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100021528","name":"Advancing a Healthier Wisconsin Endowment","doi-asserted-by":"publisher","award":["5520879"],"award-info":[{"award-number":["5520879"]}],"id":[{"id":"10.13039\/100021528","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The role of the immune system in the development of hypertension is well\u2010established. Recent studies revealed the critical role of G\u2010protein\u2010coupled receptor signaling, particularly angiotensin II (Ang II) type 1 receptor (AT1R), in immune cell function. Regulator of G\u2010protein signaling (RGS) proteins are critical players in terminating G\u2010protein\u2010coupled receptor signaling, with RGS2 being particularly interesting because it limits signaling downstream of AT1R. Importantly, RGS2 is highly expressed in myeloid immune cells. Global RGS2 deletion results in elevated blood pressure and Ang II\u2010dependent vascular hypercontractility. While evidence supports a key role for RGS2 in vascular tissue, its role in immune cells remains underexplored. Herein, we hypothesized that genetic ablation of RGS2 in myeloid cells would amplify Ang II signaling, thereby exacerbating the inflammatory response to Ang II. To test this hypothesis, we employed a conditional myeloid\u2010specific RGS2 knockout mouse model (RGS2\n                    <jats:sup>LysM\u2010KO<\/jats:sup>\n                    ) subjected to chronic Ang II treatment. Contrary to our expectations, both Control and RGS2\n                    <jats:sup>LysM\u2010KO<\/jats:sup>\n                    exhibited comparable changes in blood pressure, cardiac hypertrophy, albuminuria, renal tubular injury markers, vascular dysfunction, and inflammatory profiles in response to Ang II. Altogether, we conclude that myeloid RGS2 does not significantly affect blood pressure nor modulate renal injury or vascular dysfunction effects of Ang II.\n                  <\/jats:p>","DOI":"10.14814\/phy2.71023","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T05:52:52Z","timestamp":1784526772000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Conditional ablation of\n                    <scp>RGS2<\/scp>\n                    in myeloid cells does not influence blood pressure and organ damage in angiotensin\n                    <scp>II<\/scp>\n                    \u2010induced hypertension in mice"],"prefix":"10.14814","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1887-7367","authenticated-orcid":false,"given":"Pablo","family":"Nakagawa","sequence":"first","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Cardiovascular Center, Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Neuroscience Research Center, Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ko\u2010Ting","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Reho","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Comprehensive Rodent Metabolic Phenotyping Core Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina","family":"Ghobrial","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathren","family":"Kaminski","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Hantke\u2010Guixa","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia M.","family":"Mathieu","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel T.","family":"Brozoski","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisita","family":"Chaihongsa","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey K.","family":"Wackman","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil R.","family":"Rajendiran","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Dhoondia","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey L.","family":"Segar","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Department of Pediatrics Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1453-0921","authenticated-orcid":false,"given":"Curt D.","family":"Sigmund","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Cardiovascular Center, Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Neuroscience Research Center, Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin L.","family":"Grobe","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Cardiovascular Center, Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Neuroscience Research Center, Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Comprehensive Rodent Metabolic Phenotyping Core Medical College of Wisconsin  Milwaukee Wisconsin USA"},{"name":"Department of Biomedical Engineering Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.10.002"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2007.06.004"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(73)80047-5"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2018.05.074"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.109.144964"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199706000-00011"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.158043"},{"key":"e_1_2_11_9_1","volume-title":"Hypertension Prevalence, Awareness, Treatment, and Control Among Adults Age 18 and Older: United States, August 2021\u2013August 2023","author":"Fryar C. D.","year":"2024"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.119.14240"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.57.3.460"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070657"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2010.01.006"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2007.038240"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.10145"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI15598"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.70093"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.114.03663"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-017-1950-x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(01)00141-8"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI74084"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00060.2009"},{"issue":"9","key":"e_1_2_11_23_1","article-title":"The causal relationship between Endothelin\u20101 and hypertension: Focusing on endothelial dysfunction, arterial stiffness, vascular remodeling, and blood pressure regulation","volume":"11","author":"Kostov K.","year":"2021","journal-title":"Life (Basel)"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.121.18734"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00468.2010"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2011.01.023"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.117.032582"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.120.14972"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.220414397"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2012.03.029"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00037.2018"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.119.14056"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00170.2015"},{"key":"e_1_2_11_35_1","article-title":"Regulator of G\u2010protein signaling 2 knockout in CD4+ T cells promotes anti\u2010inflammatory T cells, enhancing ovulation, and oocyte yield","author":"Raff M.","year":"2024","journal-title":"bioRxiv"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.122.20169"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.116.06591"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.99403"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.hjh.0000226202.80689.8f"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.644903"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.034470"},{"issue":"5","key":"e_1_2_11_42_1","first-page":"2103","article-title":"Angiotensin II induces RAW264.7 macrophage polarization to the M1\u2010type through the connexin 43\/NF\u2010\u03baB pathway","volume":"21","author":"Wu L.","year":"2020","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00969.2004"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.111.237198"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/332411a0"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.879693"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199100177-00147"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00026.2011"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.71023","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.14814\/phy2.71023","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.71023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T15:03:31Z","timestamp":1786547011000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.71023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":47,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.14814\/phy2.71023"],"URL":"https:\/\/doi.org\/10.14814\/phy2.71023","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"value":"2051-817X","type":"print"},{"value":"2051-817X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71023"}}